| 青蒿组织培养的研究 |
| |
| 引用本文： | 黄红芳,刘德华,王丹,欧阳华君,陈则夷.青蒿组织培养的研究[J].湖南农业科学,2012(7):115-118. |
| |
| 作者姓名： | 黄红芳 刘德华 王丹 欧阳华君 陈则夷 |
| |
| 作者单位： | 湖南农业大学园艺园林学院,湖南长沙,410128 |
| |
| 摘 要： | 以青蒿茎段为外植体材料进行组织培养研究,结果表明:0.2%的HgCl2浸泡10 min对青蒿茎段材料灭菌有较好的效果;具腋芽的外植体在MS+6-BA(0.5～1.5)mg/L、MS+(0.01～0.05)mg/L TDZ、MS+1.0 mg/L 6-BA+0.2 mg/L IBA培养基上培养,腋芽都能萌发生长,在MS+(0.01～0.05)mg/L TDZ、MS+1.0 mg/L 6-BA+0.2 mg/L IBA培养基上培养,能诱导茎段产生大量不定芽;具有腋芽基部周围细胞的茎外植体在MS+0.5 mg/L 6-BA、MS+(0.01～0.05)mg/L TDZ培养基上培养,在其原腋芽处产生了大量丛芽。
|
| 关 键 词： | 青蒿 组织培养 外植体 不定芽 |
Study on Tissue Culture of Artemisia annua |
| |
| HUANG Hong-fang
,
LIU De-hua
,
WANG Dan
,
OUYANG Hua-jun
,
CHEN Ze-yi.Study on Tissue Culture of Artemisia annua[J].Hunan Agricultural Sciences,2012(7):115-118. |
| |
| Authors: | HUANG Hong-fang LIU De-hua WANG Dan OUYANG Hua-jun CHEN Ze-yi |
| |
| Institution: | (College of Horticulture and Landscape,HNAU,Changsha 410128,PRC) |
| |
| Abstract: | The stem of Artemisia annua was taken as explant to conduct tissue culture.The results showed that immersion of Artemisia annua stem in 0.2% HgCl2 for 10 min had better sterilization effect;culture of explant with axillary bud on media of MS+6-BA(0.5~1.5) mg/L,MS+TDZ(0.01~0.05) mg/L,MS+1.0 mg/L 6-BA + 0.2 mg/L IBA,the axillary bud can germinate and grow,on media of MS+TDZ(0.01~0.05) mg/L and MS+1.0 mg/L 6-BA+0.2 mg/L IBA can induce a large number of adventitious buds on stem;culture of explant with axillary bud basal cells on media of MS+6-BA 0.5 mg/L and MS+TDZ(0.01~0.05) mg/L,a large number of clumpy buds germinated from the position of original axillary bud. |
| |
| Keywords: | Artemisia annua tissue culture explant adventitious buds |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
