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| 高效液相色谱法检测水产品中的孔雀石绿 | |
| 引用本文： | 万译文,洪波,曾春芳,刘丽,肖维,黄华伟. 高效液相色谱法检测水产品中的孔雀石绿[J]. 湖南农业科学, 2012, 0(5): 102-104 |
| 作者姓名： | 万译文 洪波 曾春芳 刘丽 肖维 黄华伟 |
| 作者单位： | 1. 农业部渔业产品质量监督检验测试中心(长沙),湖南长沙410153;湖南省水产科学研究所,湖南长沙410153 2. 农业部渔业产品质量监督检验测试中心(长沙),湖南长沙,410153 3. 湖南省水产科学研究所,湖南长沙,410153 |
| 基金项目： | 农业部长江中上游渔业资源环境科学观测实验站开放课题 |
| 摘 要： | 采用高效液相色谱法检测水产品中的农药残留孔雀石绿。样品中的孔雀石绿经乙腈与乙酸铵提取,浓缩后固相萃取柱净化、硼氢化钾还原、反向色谱柱分离,再使用荧光检测器检测。应用该方法孔雀石绿的加标回收率在75.1%～95.1%之间,相对标准偏差3.7%～7.1%,检出限均为0.5μg/kg。结果表明:该方法检测孔雀石绿处理简单快捷,灵敏度高,可用于大量样品的快速分析。 |
| 关 键 词： | 孔雀石绿 水产品 高效液相色谱法 |
Determination of Malachite Green in Fishery Products by HPLC |
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| WAN Yi-wen , HONG Bo , ZENG Chun-fang , LIU Li , XIAO Wei , HUANG Hua-wei. Determination of Malachite Green in Fishery Products by HPLC[J]. Hunan Agricultural Sciences, 2012, 0(5): 102-104 | |
| Authors: | WAN Yi-wen HONG Bo ZENG Chun-fang LIU Li XIAO Wei HUANG Hua-wei |
| Affiliation: | 1.Fishery Products Quality Supervision and Testing Center,Ministry of Agriculture,Changsha 410153,PRC; 2.Hunan Fisheries Science Institute,Changsha 410153,PRC) |
| Abstract: | Malachite green,a kind of pesticide residues,in fishery products were determined by high performance liquid chromatography(HPLC).The malachite green residues in samples were extracted by acetonitrile and ammonium acetate,and then were purified by solid-phase extraction column after concentrated,and then were deoxidized by potassium borohydride and separated by RP-HPLC column,in the end were detected by fluorescence detector.If using this method,the standard addition recovery of malachite green was 75.1 %~95.1%,the relative standard deviation(RSD) were 3.7%~7.1%,and the detection limit was 0.5 μg/kg.Therefore,this method is an easy,quick and high sensitive method to detect malachite green,which can be used to quickly analyze mass samples. |
| Keywords: | malachite green aquatic products HPLC |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |