应用链脲左菌素构建SD大鼠2型糖尿病模型效果的研究

将20只SD雌性大鼠随机分为模型组和对照组,模型组(n=12只)喂高糖高脂饲料,对照组(n=8只)喂基础饲料。大鼠喂养4周后,以小剂量链脲佐菌素(STZ,35 mg/kg)腹腔注射,建立2型糖尿病大鼠模型。结果表明:与对照组相比,模型组血清胰岛素和空腹血糖明显上升(p<0.01),胰岛细胞数目减少,细胞质变淡。高脂喂养联合STZ(35 mg/kg)诱导大鼠2型糖尿病的方法有效且造模长期稳定、成功率高等特点,是人类2型糖尿病及其药物研究的理想动物模型。

Construction of Type 2 Diabetic SD Rat Model by Streptozotocin

Twenty SD female rats were randomly divided into two groups,control group and model group.In model group(n = 12),the rats were fed with high-fat and high-sugar pellet,and in control group(n = 8),the rats were fed with commercial standard pellet.Four weeks after feeding,intraperitoneal injection of low dosage of streptozotocin(STZ,35 mg/kg) was conducted to rats in both groups in order to build type 2 diabetic rat models.The results indicated that in comparison with control,the serum insulin levels and the fasting blood glucose(FBG) in model group all increased significantly(P<0.01),the β-cell number decreased and the cytoplasm became light.The method that inducing type 2 diabetic rat by combining high-fat and high-sugar feeding with intraperitoneal injection of STZ with 35 mg/kg is effective,and the models built by the method are stable for a long period of time and with high success rate.Therefore,the models built by the method are the ideal animal model for studying human type 2 diabetes mellitus and its drug.