藤茶植株叶果与组培物中二氢杨梅素含量的比较研究

为比较藤茶经组织培养后不同组织中二氢杨梅素的含量,采用高效液相色谱法(HPLC)测定藤茶叶片、果实、悬浮培养液、嫩叶、愈伤组织、老叶中二氢杨梅素的含量。结果表明:嫩叶、叶片、老叶、果实、愈伤组织、悬浮培养液中二氢杨梅素的含量分别为:25.528%、13.709%、13.337%、6.255%、11.616%、0.829%。愈伤组织中二氢杨梅素含量与叶片接近,悬浮液中含量最低,可以尝试运用愈伤组织培养法生产二氢杨梅素。

Compare of Contents of Dihydromyricetin in Leaf, Fruit and Tissue Culture Objects of Ampelopsis grossedentata

In order to compare the contents of dihydromyricetin in leaf,fruit and tissue culture objects of Ampelopsis grossedentata,the content of dihydromyricetin in immaturate leaf,mature leaf,post-mature leaf,fruit,suspension cultures,callus of Ampelopsis grossedentata were determined by HPLC.The results showed that the contents of dihydromyricetin in immaturate leaf,mature leaf,post-mature leaf,fruit,suspension culture solution,callus of Ampelopsis grossedentata were 25.528%,13.709%,13.337%,6.255%,0.829% and 11.616%,respectively.The content of dihydromyricetin in mature leaf was similar to that in callus,and the content of dihydromyricetin in suspension culture solution was the lowest.It is a try to produce dihydromyricetin through callus culture method in future.