不同引物组合对中国杏品种资源S基因特异扩增效果比较

 利用已报道的5对蔷薇科李属通用引物组合对起源于我国的30个杏(Prunus armeniaca L.)品种进行了S等位基因的专一性PCR扩增（S-allele specific PCR），并依据扩增系数和扩增成功率两个指标对扩增效果进行了评价。结果表明：①不同的引物组合对参试杏品种的扩增系数和扩增成功率差异很大，其中引物组合EM PC2consFD+EM PC3consRD扩增系数和扩增成功率均为最高（66.7%和53.3％），效果最好；②引物组合EM PC2consFD+EM PC3consRD对‘华县大接杏’和‘猪皮水杏’未能扩增出任何条带，而PruC2+PruC4R和PaConsⅡ F+PaConsⅡ R两对组合却能对其扩增出两条带；此外，5对组合均没有对‘兰州大接杏’和‘白木杏’这两个品种扩增出任何条带，表明要对中国杏品种资源的S基因型进行全面、准确鉴定，除了采用不同的引物组合外，还需要根据中国杏的S基因序列进一步开发、设计新的专用引物；③ EM-PC2consFD+EM PC3consRD和PruC2+PruC4R两对组合对‘红丰’、‘新世纪’、‘红荷包’和‘二花槽’等4个品种的扩增结果表明，依据本试验所提出的扩增系数和扩增成功率两个指标评价不同引物组合的扩增效果是有效的。

Comparison of Amplification Effects on Apricot (Prunus armeniaca L.) Resources in China Using Different Primer Combinations

S genotypes of 30 apricot cultivars native to China were detected usingS-allele-specific PCR with 5 pairs of Prunus consensus primer combinations which had been published, and amplification effects were evaluated according to coefficient and efficiency of amplification. The results were as follows: ①The effects of PCR amplification with 5 primer combinations were different greatly. The primer combination EM-PC2consFD+EM-PC3consRD was the most suitable for Chinese apricot, and its’ coefficient and efficiency of amplification were 66.7% and 53.3%, respectively; ②No amplification was obtained using the combination EM-PC2consFD+EM-PC3consRD in the two cultivars ‘Huaxian Dajiexing’ and ‘Zhupi Shuixing’ while the other two combinations, PruC2+PruC4R and PaConsⅡ-F+PaConsⅡ-R, could amplify two fragments: In addition, no amplification was obtained using all the five pairs of combinations in the two cultivars ‘Lanzhou Dajiexing’ and ‘Baimuxing’. Then it could be concluded that, to detect S genotypes of all Chinese apricot cultivars accurately, new specific primers need to be developed besides the five primer combinations; ③The results amplified in the four cultivars ‘Hongfeng’,‘Xinshiji’, ‘Honghebao’ and ‘Erhuacao’ by two pairs of combinations, EM-PC2consFD+EM-PC3consRD and PruC2+PruC4R, showed that it was of validity to evaluate amplification effects of different primer combinations according to coefficient and efficiency of amplification in this study.