| H5N1亚型禽流感病毒神经氨酸酶基因的克隆及序列分析 |
| |
| 引用本文： | 王振国,金宁一,郑敏,马鸣潇,张洪勇,尹革芬.H5N1亚型禽流感病毒神经氨酸酶基因的克隆及序列分析[J].吉林农业大学学报,2004,26(2):213-216. |
| |
| 作者姓名： | 王振国 金宁一 郑敏 马鸣潇 张洪勇 尹革芬 |
| |
| 作者单位： | 解放军军需大学军事兽医研究所,长春,130062;吉林出入境检验检疫局检验检疫技术中心,长春,130062;解放军军需大学军事兽医研究所,长春,130062 |
| |
| 基金项目： | 吉林省科技发展基金资助项目 |
| |
| 摘 要： | 应用RT-PCR方法，以1株H5N1亚型禽流感病毒RNA为模板，扩增了NA全基因cDNA。将NA cDNA克隆于pMD18-T栽体，并对其进行了测序。测序结果表明：所克隆的NA基因全长为1416bp，编码区为1410个核苷酸，编码469个氨基酸。将其序列与5株H5N1亚型禽流感病毒NA基因序列进行比较，其核苷酸和氨基酸同源性均为96.6％～99．1％。NA的克隆和分子进化分析揭示了各毒株间的亲源关系。
|
| 关 键 词： | RT-PCR 禽流感病毒 NA基因 序列分析 |
| 文章编号： | 1000-5684(2004)02-0213-04 |
Cloning and Sequencing of Neuraminidase Gene of an AIV Isolate(H5N1) |
| |
| WANG Zhen-guo,JIN Ning-yi,ZHENG Min,MA Ming-xiao,ZHANG Hong-yong,YIN Ge-fen.Cloning and Sequencing of Neuraminidase Gene of an AIV Isolate(H5N1)[J].Journal of Jilin Agricultural University,2004,26(2):213-216. |
| |
| Authors: | WANG Zhen-guo JIN Ning-yi ZHENG Min MA Ming-xiao ZHANG Hong-yong YIN Ge-fen |
| |
| Abstract: | In this study, full-length neuraminidase(NA) gene was amplified from an avian influenza virus isolate(H5N1) by RT - PCR. The cDNA of NA gene was cloned into pMD18-T vector and then was se-quenced. The cloned 1 416 bp fragment covered the whole NA gene including the completely open reading frame which encoded 469 amino acids. The results of comparative sequence analysis indicated that the nucleotide sequence of NA gene of this isolate has 96.6 % ~ 99.1 % homology with those of other three H5 N, 1 isolates. The putative amino acid sequence has 96. 6 % ~ 99.1 % homology with those of other five H5 N1 isolates. This study revealed the relationship among these isolates. |
| |
| Keywords: | RT - PCR avian influenza virus neuraminidase gene sequencing |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
