| 10株火鸡组织滴虫18S rRNA基因的克隆及进化分析 |
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| 引用本文： | 刘进辉,彭俊宇,宋海燕,刘毅. 10株火鸡组织滴虫18S rRNA基因的克隆及进化分析[J]. 畜牧兽医学报, 2011, 42(3): 448-452 |
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| 作者姓名： | 刘进辉 彭俊宇 宋海燕 刘毅 |
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| 作者单位： | 湖南农业大学动物医学院,长沙,410128 |
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| 基金项目： | 国家自然科学基金(30771616); 长沙科技计划一般项目(K0902144-21)资助 |
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| 摘 要： | 应用聚合酶链反应(PCR)鉴定湖南娄底(简称HMLD1-3)、湘潭(HMXT1-2)、永州(HMYZ1-3)和宁乡(HMNX1-2)共10株火鸡组织滴虫,并用18S rRNA部分序列重构火鸡组织滴虫与其它相近原虫的种群遗传关系.作者通过PCR扩增火鸡组织滴虫虫株的18S rRNA部分序列,将PCR扩增出的片段纯化后克隆...
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| 关 键 词： | 火鸡组织滴虫 核糖体18S rRNA 序列分析 种系发育关系 |
Cloning and Phylogenetic Analysis of the 18S rRNA of Histomonas meleagridis Isolates from Hunan Province |
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| LIU Jin-hui,PENG Jun-yu,SONG Hai-yan,LIU Yi. Cloning and Phylogenetic Analysis of the 18S rRNA of Histomonas meleagridis Isolates from Hunan Province[J]. Chinese Journal of Animal and Veterinary Sciences, 2011, 42(3): 448-452 |
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| Authors: | LIU Jin-hui PENG Jun-yu SONG Hai-yan LIU Yi |
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| Affiliation: | LIU Jin-hui,PENG Jun-yu,SONG Hai-yan,LIU Yi (College of Veterinary Medicine,Hunan Agricultural University,Changsha 410128,China) |
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| Abstract: | Histomonas meleagridis were identified by PCR form Hunan Province,and their phylogenetic relationship was analysed by using 18S rRNA sequences.The 18S rRNA sequences were amplified from each H.meleagridis sample and the amplicons were cloned into pGEM-T Easy vector,respectively.The inserts were successfully sequenced,and the length of 18S rRNA of the 10 H.meleagridis isolates were 532 bp.Sequence comparison revealed that the similarity in18S rRNA sequences between Hunan isolates and the H.meleagridis availa... |
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| Keywords: | Histomonas meleagridis 18S rRNA sequence analysis phylogenetic relationship |
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