| 猪TLR3基因A1116T点突变功能初步分析 |
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| 引用本文： | 邢明伟,翟春媛,李海涛,杨秀芹.猪TLR3基因A1116T点突变功能初步分析[J].畜牧兽医学报,2011,42(4). |
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| 作者姓名： | 邢明伟 翟春媛 李海涛 杨秀芹 |
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| 作者单位： | 1. 东北林业大学野生动物资源学院,哈尔滨,150040
2. 东北农业大学动物科技学院,哈尔滨,150030 |
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| 基金项目： | 中国博士后科学基金(20080430950); 黑龙江省自然科学基金(200927) |
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| 摘 要： | 为了确定猪TLR3基因A1116T错义突变的生物学效应,研究其与猪疾病抗性/易感性的关系,本研究采用重叠延伸PCR和定点突变技术构建不同等位基因的真核表达载体,采用双荧光素酶检测分析系统及Real-timePCR方法在体外培养的细胞中研究不同等位基因在信号转导中的作用。成功地构建了野生型和突变型TLR3的真核表达载体,双荧光素酶检测分析表明突变型活化转录因子NF-κB、激活ISRE的能力都减弱;Real-time PCR分析表明突变型诱导转录IL-6的能力下降。结果表明该点突变影响猪TLR3的信号转导能力。
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| 关 键 词： | 猪 TLR3基因 突变 信号转导 |
A11116T SNP Functional Analysis of Porcine TLR3 Gene |
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| XING Ming-wei,ZHAI Chun-yuan,LI Hai-tao,YANG Xiu-qin.A11116T SNP Functional Analysis of Porcine TLR3 Gene[J].Acta Veterinaria et Zootechnica Sinica,2011,42(4). |
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| Authors: | XING Ming-wei ZHAI Chun-yuan LI Hai-tao YANG Xiu-qin |
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| Institution: | XING Ming-wei1,ZHAI Chun-yuan2,LI Hai-tao2,YANG Xiu-qin2 (1.College of Wildlife Resources,Northeast Forestry University,Harbin 150040,China,2.College of Animal Science and Technology,Northeast Agricultural University,Harbin 150030,China) |
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| Abstract: | In order to reveal the biological role of nonsynonymous SNP A1116T of porcine TLR3 gene and to investigate the relationship of the SNP with disease resistance/susceptibility,the study was designed to construct eukaryotic expression vector of two types of alleles by splicing overlap extension PCR and site-directed mutation methods,and to analyze the function of the two alleles in signal transduction in cultured PK-15 by dual-luciferase reporter assay and Real-time PCR methods.Two types of eukaryotic expressi... |
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| Keywords: | porcine TLR3 mutation signal transduction |
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