| 梅PGIP基因的克隆及全序列分析 |
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| 引用本文： | 李广平,房经贵,蔡斌华,章镇,张长青.梅PGIP基因的克隆及全序列分析[J].园艺学报,2006,33(1):125-127. |
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| 作者姓名： | 李广平 房经贵 蔡斌华 章镇 张长青 |
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| 作者单位： | ( 南京农业大学园艺学院, 南京210095; 金陵科技学院园艺系, 南京210038) |
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| 摘 要： | 通过PCR扩增, 从梅基因组中得到1条全长1 192 bp的多聚半乳糖醛酸酶抑制蛋白( PGIP)基因序列。该序列包含有1个完整的开放阅读框和1个内元。比对结果表明, 克隆到的序列与桃、马哈利樱桃中相应序列一致度分别为96%和95% , 其蛋白质序列与桃、马哈利樱桃的蛋白质序列一致度分别为97%和94%。该蛋白质序列中包含着一段亮氨酸重复序列。
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| 关 键 词： | 梅 PGIP基因 克隆 序列分析 |
| 文章编号： | 0513-353X（2006）01-0125-03 |
| 收稿时间： | 2004-12-08 |
| 修稿时间： | 2004-12-082005-05-12 |
Cloning and Sequencing of PGIP Gene from Prunus mume Sieb |
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| Li Guangping,Fang Jinggui,Cai Binhua,Zhang Zhen,Zhang Changqing.Cloning and Sequencing of PGIP Gene from Prunus mume Sieb[J].Acta Horticulturae Sinica,2006,33(1):125-127. |
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| Authors: | Li Guangping Fang Jinggui Cai Binhua Zhang Zhen Zhang Changqing |
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| Institution: | (College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China; Department of Horticulture, Jinling Institute of Technology, Nanjing 210038, China) |
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| Abstract: | A 1 192 bp sequence of polygalacturonase-inhibiting protein(PGIP)gene(Genbank locus:AY764131) was isolated by polymerase chain reaction(PCR) from Prunus mume.This sequence had a(intron) and a full open reading frame encoding the polygalacturonase-inhibiting protein.Its gene and derived protein were both highly homologous to those from Prunus persica and Prunus mahaleb.A conserved leucine-rich fragment had existed in the derived protein sequence. |
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| Keywords: | Prunus mume PGIP gene Cloning Sequencing |
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