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南美白对虾几丁质酶的分离纯化
引用本文:马盛群.南美白对虾几丁质酶的分离纯化[J].金陵科技学院学报,2003,19(4):44-46.
作者姓名:马盛群
作者单位:金陵科技学院动科系,江苏,南京,210008
摘    要:采用先“盐析”后“亲和吸附”的方法分离虾几丁质酶,纯化倍数为15.3倍,比活率73.3ImU·mg-1,产品得率50.6%;亲和分离胶为商品化的脱乙酰几丁质经再生后制备而成。方法简单易行适于放大生产。

关 键 词:南美白对虾  几丁质酶  亲和纯化
文章编号:1008-1895(2003)04-0044-03
修稿时间:2003年9月6日

Purification of Chitinase from Penaeus Vannamei
MA Shen-qun.Purification of Chitinase from Penaeus Vannamei[J].Journal of Jinling Institute of Technology,2003,19(4):44-46.
Authors:MA Shen-qun
Abstract:This method of purificating chitinase from shrimp by salting out and affinity absorbent was reported here. The puritic coefficient was 15.3 fold;specific activity was 73.3 ImU.mg-1; and the productivity was 50.6% . Affinity absorbent gel was specially made by the chitinase. This method is well suited for large scale production.
Keywords:penaeus vannamei  chitinase  affinity purification
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