鲟鱼硫酸软骨素的纯化及其特性

采用乙醇分级沉淀、柱层析等方法纯化了鲟鱼硫酸软骨素,并对提取纯化的鲟鱼硫酸软骨素(色谱纯)与Sigma的鲨鱼硫酸软骨素(色谱纯)进行了比较,探讨了二者在分子量、旋光度、硫酸基团含量和紫外可见光谱等方面的差异.结果表明,鲟鱼硫酸软骨素的重均、数均和Z均分子量、比旋光度、硫酸基团含量均与鲨鱼硫酸软骨素存在显著差异(P<0.05);在紫外可见光谱图中,鲟鱼硫酸软骨素的最大吸收波长在220nm,而鲨鱼硫酸软骨素则在230nm.这些差异表明,鲟鱼硫酸软骨素在生物活性和药用功能方面可能与鲨鱼硫酸软骨素存在区别,并推测鲟鱼硫酸软骨素可能具有较好的抗氧化活性和抗凝血活性.

Purification and characteristics of sturgeon chondroitin sulfate

Condroitin sulfate (CS) has many functions and is widely used in food, medical and cosmetic industies. Elasmobranch is the main material source of fish chondroitin sulfate. Nowadays the main fish condroitin sulfate product is from shark cartilage. However, it can not be produced greatly for the limited resource of shark. Sturgeon has resource advantage because it can be fostered by manpower. But the characteristics of sturgeon chondroitin sulfate has not been learned so far. In the present paper, sturgeon chondroitin sulfate was purified by the methods of ethanol classification precipitation and column chromatography. The purified sturgeon CS (chromatographically pure) and shark CS (chromatographically pure) of Sigma company were comparatively studied in molecular weight, rotation, sulfate group content, UV and visible spectra. The results show that weight average, number average and Z average molecular weights of sturgeon CS are remarkably different from those of shark CS (P<0.05). Molecular weight distribution indicates that the purity of extracted sturgeon CS is more higher than the shark CS (P<0.05). It also shows that the sulfate group content of sturgeon CS is less than that of shark CS(P<0.01). The power rotations of the two CSs are proved to be very different, too (P<0.01). The strongest absorbance wavelength was 220 nm in UV and visible spectrum of sturgeon CS, while 230 nm in shark CS. These characteristics of sturgeon CS suggest its probable speciality in bioactivity and medical function. Presumably, sturgeon CS may have better anti-oxidation and anti-coagulant activities.