The myrobalan (<Emphasis Type="Italic">Prunus cerasifera</Emphasis> L.): a useful diploid model for studying the molecular genetics of self-incompatibility in plums |
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Authors: | B G Sutherland R Cerovič T P Robbins K R Tobutt |
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Institution: | (1) East Malling Research, New Road, ME19 6BJ East Malling, Kent, UK;(2) Plant Sciences Division, School of Bioscience, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, UK;(3) Calle Tómas de Aquino 4-5B, 14004 Cordoba, Spain;(4) Fruit and Grape Research Centre, Cralja Petra I, 32000 Cacak, Serbia |
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Abstract: | A series of PCR methods were used to detect S-RNase alleles and SFB alleles and to determine S-genotypes in 25 accessions of myrobalan (Prunus cerasifera L.). Firstly, primers flanking the polymorphic second intron were used to identify S-RNases in agarose gels. These primers amplified one or two bands per accession in 25 accessions. Then consensus primers were designed
for amplifying the polymorphic first intron, unique to Prunus S-RNases, for automated fluorescent detection. Each accession produced one or two peaks. New primers were then developed to amplify
the intron in the SFB gene, for detection by fluorescence. Cross-referencing PCR bands and peaks indicated 15 S-alleles were present in the 25 accessions. Cloning, sequencing and comparison with published data indicated that the amplified
products were S-RNase alleles. Sequence information was used to design primers specific for each S-RNase. Full and consistent S-genotypes were obtained by cross-comparing PCR data for 23 of the 25 accessions, and two accessions appeared to have a single
allele. Pollen-tube microscopy indicated function of some but not all of the S-alleles sequenced. |
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Keywords: | Myrobalan Prunus cerasifera S-RNase SFB S-genotyping |
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