杨树部分种的AFLP遗传多样性分析

利用10对AFLP引物对杨属5个派20个种及杂种的44份杨树材料进行分析.结果表明:在这些材料中,不同引物组合的多态性均为100%,表明杨属种间及无性系间在DNA 水平上存在广泛变异.利用 UPGMA 法对 AFLP 数据进行聚类分析,相似系数在 0.765～0.971 之间,派间聚类与经典形态分类完全一致,派内种间及种内无性系间聚类与形态分类基本相同.构建了 44 份材料的指纹图谱,在该图谱中,每个材料都具有自身独特的条带.最后,探讨根据分子标记结果进行杂交亲本选配及杂种子代早期选择的可行性.

Analysis of Genetic Diversity of Some Species in Populus by AFLP Marker

Ten pairs of AFLP primers were screened out and used for the analysis of forty-four clones, which came from twenty species and hybrids of five sections in Populus. Percentage of polymorphism fragments produced by each pair of AFLP primer was 100%. All these data indicated that considerable genetic variation existed among species and clones at DNA level. Using UPGMA cluster analysis from AFLP data,molecular genetic distances among sections, species, and clones were calculated, and the relationship among them was described quantitatively. Compared with the classical taxonomy, the result of cluster analysis among sections was consistent completely, and the results of cluster analysis among intrasectional species and intraspecies clones were basically similar. We built DNA fingerprint of the 44 Populus genotypes. In this fingerprint, every plant has its own band. Finally, the feasibility of selecting and mating parents and early selection of progenies were discussed based on the results of molecular markers.