不同来源辣椒疫霉菌的遗传多样性分析

目的]探索来自辣椒寄主和土壤的辣椒疫霉的遗传多样性。方法]通过利用12个10碱基随机引物对来自我国4个不同地理区域的22个辣椒疫霉菌株的亲缘关系进行RAPD分析。结果]受试22个菌株共产生101条谱带,其中多态性为99条,占98.02%,说明受试辣椒疫霉菌具有丰富的遗传多样性。根据引物扩增的DNA指纹图谱,运用UPGMA分析法,以遗传相似系数0.5为阈值,将供试22个菌株划分为3个遗传聚类组(Ⅰ、Ⅱ、Ⅲ)。RAPD标记技术分析表明,来辣椒寄主和土壤的菌株的全基因组DNA扩增图谱差异很大。结论]供试菌株具有丰富的遗传多样性,来自不同遗传背景的菌株差异显著,聚类组的划分与菌株的来源有一定的相关性。

Analysis of Genetic Diversity of Phytophthora capsici from Different Sources

Objective] The research aimed to explore the genetic diversity of Phytophthora capsici from pepper host and soil.Method] Through using twelve 10-base random primers,the genetic relationships of 22 strains of Phytophthora capsici from 4 different areas in China were analyzed by RAPD.Result] 22 tested strains produced 101 spectral bands,in which polymorphisms were 99,accounting for 98.02%,which indicated that the abundant genetic diversities existed among the tested Phytophthora capsici.By using UPGMA analysis,with the genetic similarity coefficient 0.5 as the threshold,the 22 Phytophthora capsici were classified into three cluster groups(I,II,III) according to the DNA fingerprints spectrum of primer amplification.The RAPD marker technology showed that large differences existed in the DNA amplified spectrum of complete genome of strains from pepper host and soil.Conclusion] The tested strains had abundant genetic diversities.Strains from different genetic backgrounds were notably different.There was certain relationship between the classification of cluster groups and the sources of strains.