甘蔗种质遗传基础的AFLP分析

采用AFLP分子标记技术对54份甘蔗种质（14份祖亲种、40份栽培品种或品系）的遗传基础进行了分析。利用筛选出的4对多态性较强的引物组合（M+CAG/E+ACG，M+CTC/E+ACT，M+CTG/E+ACC，M+CTG/E+ACG），构建了甘蔗54份种质的AFLP指纹图谱，这4对引物组合共扩增出396条谱带，其中多态带390条，占98.5%。54份种质的遗传相似系数变化

AFLP Analysis for Sugarcane Germplasms

Modern sugarcane cultivars (Saccharum spp.) are polyploid or aneuploid clones with complex genetic background, and the major components of the genome derived from S. officinarum, and the remainder from S. spontaneum, S. barberi, S. robustum, S. sinense, and Erianthus arundinaceus. In this study, 54 accessions of sugarcane germplasms (14 parental species, 40 cultivars or clones) were analyzed by amplified fragment length polymorphism (AFLP). Four primer pairs, i.e., M CAG/E ACG, M CTC/E ACT, M CTG/E ACC and M CTG/E ACG, were selected and used to establish the AFLP fingerprints of the germplasms. There were 396 bands, of which 390 were polymorphic, accounting for 98.5%. The AFLP data were clustered using UPGMA method. The genetic similarity coefficients among 54 germplasms ranged from 0.281 to 0.943, with a mean of 0.708. Based on the difference of the similarity coefficients, the sugarcane germplasms could be distinguished among inter-genus(Saccharum and Eranthus arundinaceus), wild species(S. spontaneum and S. robustum ), cultivated species(S. officinarum, S. barberi and S. sinense ), cultivated species and cultivars derived from interspecific hybridization. The similarity between cultivars and parental species S. officinarum were the highest, and followed by S. barberi, S. sinense, S. robustum, S. spontaneum and E. arundinaceus in the order from high to low. Clustering based on AFLP showed that the groupings of most cultivars were corresponded to their pedigrees, in which a given cultivar and one of its parents was always clustered into the same group. For example, the cultivar Co 290 and its hybrid offspring such as Co 419, F134, Mintang 70/611, Guangdong 7, Yuetang 71/210, Yuetang 64/395, Guangxi 1, Yacheng 62/40 and Haizhe 4 etc., were clustered into the group D(see Fig.2). The relationship between S. barberi and S. sinense was the closest among the parental species. E. arundinaceus was distantly related to Saccharum.