肉鸭PLIN基因PCR RFLP多态性与胴体、脂肪性状关联分析

研究PLIN基因外显子2和内含子2多态性与肉鸭部分产肉性状的关系。以361只北京鸭(Z2系、Z4系、Z2×Z4杂交系)和樱桃谷鸭为材料,利用PCR-RFLP方法和测序技术,对PLIN基因外显子2和内含子2进行SNPs位点检测,并对SNPs位点与部分产肉性状进行关联性分析。结果显示,在外显子2检测到C→T突变,分析表明,樱桃谷鸭群体中,胴体质量、全净膛质量和腹脂质量分数在CC和CT型之间差异极显著(P<0.01),腹脂质量和皮脂质量分数在CC和CT型之间差异显著(P<0.05);北京鸭Z4系群体中,全净膛质量在TT型与CC、CT型之间差异显著(P<0.05);北京鸭杂交系群体中,全净膛质量在CC和CT型之间差异显著(P<0.05)。在内含子2检测到C→T突变,分析表明,北京鸭Z4系群体中,全净膛质量在EE和DE型之间差异极显著(P<0.01),EE和DD型之间差异显著(P<0.05);北京鸭杂交系群体中,胸肌质量在DD和DE型之间差异极显著(P<0.01),皮脂质量分数在DD和EE型之间差异显著(P<0.05)。说明PLIN基因单核苷酸多态性对肉鸭胴体、脂肪性状有一定的影响,可作为影响胴体和肉质的候选基因,指导优良肉鸭品种的选育工作。

Analysis of PLIN Gene PCR RFLP Polymorphism and Its Association with Carcass and Adipose Traits in Meat Ducks

Polymorphisms of PLIN gene exon 2 and intron 2 and their correlation with part of duck meat production traits were studied in this paper.361 Beijing ducks(Z2 strain,Z4 strain,Z2 × Z4 hybrid strain) and Cherry Valley ducks were used as materials and PCR-RFLP and sequencing methods were used for SNPs detection of exon 2 and intron 2 of PLIN gene.Correlation analysis was carried on between the discovered SNPs and part of the meat production traits in ducks.The SNP detection showed a nucleotide mutation(C→T) exists in exon 2 and the result of correlation analysis showed as follows: in Cherry Valley ducks,carcass mass,eviscerated mass and abdominal fat percentage were extremely different between the genotype CC and genotype CT(P<0.01),and abdominal fat mass and sebum rate were significantly different between CC and CT(P<0.05).In the Beijing duck Z4,there was a significant difference in eviscerated mass among genotype TT,CC,and CT(P<0.05).In Beijing duck(Z2 × Z4),there was a significant difference between CC and TT(P<0.05) in eviscerated mass and a nucleotide mutation(C→T) was detected in intron 2.The analysis showed in the Beijing duck Z4,eviscerated mass were extremely different between EE and DE genotypes(P<0.01)and significantly different between EE and DD(P<0.05).In the Beijing duck(Z2 × Z4),breast muscle mass between DD and DE was extremely different(P<0.01) and sebum rate was significantly different between DD and EE(P<0.05).This study indicated single nucleotide polymorphisms of PLIN gene have a certain influence on the duck carcass and adipose traits,thus it can be a candidate gene affecting carcass and meat quality,guiding the breeding work of good duck species.