白花泡桐羟基肉桂酰辅酶A还原酶mRNA全序列克隆及序列分析

经过对其他物种CCR mRNA序列的对比分析后,设计保守区兼并引物,首先用RT-PCR方法得到229 bp CCR mRNA部分序列,之后通过RACE(Rapid Amplification of cDNA Ends)方法,成功克隆得到包括5'UTR和3'UTR在内的CCR全部mRNA序列,并进行了分析,所得CCRmRNA全序列共1 243个碱基,CDS共999个碱基(103-1 101),编码氨基酸332,和其他多个物种的CCR序列比对结果显示相似度均在80%以上。此序列成功克隆之前,尚未见到白花泡桐木质素代谢关键酶基因的报道,这对丰富白花泡桐基因资源、有针对性的进行白花泡桐品质或材质改良有巨大的意义。

Cloning and Analysis of Cinnamoyl-CoA Reductase mRNA of Paulownia fortunei

The CCR mRNA sequence alignment was analyzed between several species of Paulownia fortunei,and the degenerated primers were designed.The partial sequence was amplified with RT-PCR,and then the 5'UTR and 3'UTR sequences were cloned with rapid amplification of cDNA Ends(RACE) method.The complete sequence was 1 243 bp,including 999 bp CDS,and 332 amino acids were coded.Before the CCR mRNA complete sequence was published,no any sequence was substrate in GenBank nucleotide sequence database about P.fortunei lign...