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| Cd2+人工抗原的制备及其抗体特性 | |
| 引用本文： | 张海棠,姜金庆,王顺来,刘耀东,王淑云,王自良.Cd2+人工抗原的制备及其抗体特性[J].西北农业学报,2011,20(6):43-47. |
| 作者姓名： | 张海棠 姜金庆 王顺来 刘耀东 王淑云 王自良 |
| 作者单位： | 1. 河南科技学院动物科学学院,河南新乡,453003 2. 河南省新乡市辉县畜牧局,河南新乡,453600 3. 河南科技学院新科学院,河南新乡,453003 |
| 基金项目： | 河南省高校科技创新人才支持计划资助项目 |
| 摘 要： | 用异硫氰酸苄基乙二胺四乙酸(ITCBE)鳌合Cd2+合成Cd-ITCBE半抗原,异硫氰酯法制备免疫抗原Cd-ITCBE-BSA,紫外分光光度法(UV)、SDS-PAGE和电感耦合等离子体质谱法(ICP-MS)进行鉴定;用Cd-ITCBE-BSA免疫Balb/C小鼠,间接ELISA测定多抗血清(pAb)效价,阻断ELISA鉴定其敏感性,交叉反应试验鉴定其特异性。结果表明,Cd-ITCBE-BSA偶联成功,Cd-ITCBE-BSA中BSA和Cd2+的含量分别为7.1g/L和191.7 mg/L;间接ELISA效价达到1∶(5.12×104),阻断ELISA检测Cd-EDTA的IC50为26.9μg/L,除与Hg-EDTA具有较强交叉反应外,与EDTA及其他金属离子鳌和物无交叉反应,获得高效价、敏感、特异的Cd2+pAb,为Cd2+残留免疫学检测方法的建立奠定基础。 |
| 关 键 词： | Cd2+ 半抗原 人工抗原 抗体 特性 |
Synthesis of Artificial Antigen and Characteristics of Polyclonal Antibody of Cadmium Ion |
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| ZHANG Haitang,JIANG Jinqing,WANG Shunlai,LIU Yaodong,WANG Shuyun and WANG Ziliang.Synthesis of Artificial Antigen and Characteristics of Polyclonal Antibody of Cadmium Ion[J].Acta Agriculturae Boreali-occidentalis Sinica,2011,20(6):43-47. | |
| Authors: | ZHANG Haitang JIANG Jinqing WANG Shunlai LIU Yaodong WANG Shuyun and WANG Ziliang |
| Institution: | ZHANG Haitang1,JIANG Jinqing1,WANG Shunlai2,LIU Yaodong1,WANG Shuyun3 and WANG Ziliang1(1.School of Animal Science,Henan Institute of Science and Technology,Xinxiang Henan 453003,China,2.Bureau of Animal Husbandry in Huixian City,Xinxiang Henan 453600,3.Xinke College,China) |
| Abstract: | Artificial hapten Cd-ITCBE was synthesized using isotrhiocyanobenzyl-EDTA(ITCBE) to chelate cadmium ion(Cd2+).The method of isothiocyanate(ITC) was used to conjugate Cd-ITCBE to bovine serum albumin(BSA) and artificial immunogen Cd-ITCBE-BSA was obtained.Ultraviolet(UV),sodium dodecyl sulfate polyacrylamide gel electropheresis(SDS-PAGE) and inductively coupled plasma mass spectrometry(ICP-MS) were used to identify Cd-ITCBE-BSA.Balb/C mice were immunized with Cd-ITCBE-BSA,the titres of polyclonal antibody(pA... |
| Keywords: | Cadmium ion Hapten Artificial immunogen Antibody Characteristics |
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