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| 气相色谱内标法测定苦马豆素的含量 | |
| 引用本文： | 赵兴华,何 欣,王建华.气相色谱内标法测定苦马豆素的含量[J].西北农业学报,2011,20(6):71-74. |
| 作者姓名： | 赵兴华 何 欣 王建华 |
| 作者单位： | 1. 河北农业大学中兽医学院,河北定州073000;西北农林科技大学动物科技学院,陕西杨凌712100 2. 河北农业大学中兽医学院,河北定州,073000 3. 西北农林科技大学动物科技学院,陕西杨凌,712100 |
| 摘 要： | 以气相色谱内标法测定变异黄芪中苦马豆素的含量。用超声波协助提取并用溶剂萃取变异黄芪样品,选用D-甘露醇为内标物,采用AT.SE-54毛细管作为色谱柱,柱温210℃,气化室300℃,检测器280℃,分流比30∶1,氢火焰离子化检测器。结果表明,苦马豆素与内标分离良好,在0.015 6~1.0 g/L时线性关系良好(R2=0.999 7),平均加样回收率为90.6%(RSD=2.41%)。该方法简便、灵敏、易操作,可用于疯草类植物样品中苦马豆素含量的检测。 |
| 关 键 词： | 苦马豆素 疯草 气相色谱 衍生化 |
Determination of Swainsonine of Astragalus variabilis Bunge by Gas Chromatography with Solvent Extraction |
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| ZHAO Xinghu,HE Xin and WANG Jianhua.Determination of Swainsonine of Astragalus variabilis Bunge by Gas Chromatography with Solvent Extraction[J].Acta Agriculturae Boreali-occidentalis Sinica,2011,20(6):71-74. | |
| Authors: | ZHAO Xinghu HE Xin and WANG Jianhua |
| Institution: | ZHAO Xinghua1,2,HE Xin1 and WANG Jianhua2(1.College of Traditional Chinese Veterinary Medicine,Agricultural University of Hebei,Dingzhou Hebei 073000,China,2.College of Animal Science and Technology,Northwest A&F University,Yangling Shaanxi 712100,China) |
| Abstract: | To develop a reliable method for determination of swainsonine of Astragalus variabilis Bunge by gas chromatography(GC),swainsonine was extracted with ultrasound-assisted liquid extraction method.AT.SE-54 capillary column was used and temperature was 210℃,injection temperature was 300℃,detector temperature was 280℃,splitting ratio was 30∶1,FID detector,the internal standard was D-Mannito.Swainsonine and D-Mannito were well separated,the standard curve was linear in the concentration range of 0.015 6-1.0 g/L(R2=0.999 7).The average recovery was 90.6%(RSD=2.41%).The method can be used to determine swainsonine of locoweed with high sensitivity,precise and good reproducibility. |
| Keywords: | Swainsonine Locoweed Gas chromatography Trimethylisilyate |
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