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| 肠艾美耳球虫河北株18S rDNA部分序列测定及系统发育分析 | |
| 引用本文： | 方素芳,崔 平,顾小龙,索 勋. 肠艾美耳球虫河北株18S rDNA部分序列测定及系统发育分析[J]. 西北农业学报, 2011, 20(5): 31-34 |
| 作者姓名： | 方素芳 崔 平 顾小龙 索 勋 |
| 作者单位： | 1. 中国农业大学动物医学院,北京,100193;河北北方学院动物科技学院,河北张家口,075000 2. 河北北方学院动物科技学院,河北张家口,075000 3. 中国农业大学动物医学院,北京,100193 |
| 基金项目： | 国家兔产业技术体系项目 |
| 摘 要： | 从河北某兔场单卵囊分离肠艾美耳球虫并接种无球虫兔进行增殖,CTAB法提取肠艾美耳球虫卵囊基因组DNA.利用艾美耳属球虫18S rDNA保守引物,PCR扩增肠艾美耳球虫18S rDNA片段,产物纯化后测序.测得的序列用DNAstar软件分析并与GenBank公布的11种兔球虫(EF694007-EF694017)的相应序列进行同源性分析,并绘制系统进化树.结果表明,扩增出的18S rDNA片段大小为1 521 bp.序列分析显示,肠艾美耳球虫河北株18S rDNA与GenBank公布的11种兔球虫相应序列同源性为92.6%～99.9%,肠艾美耳球虫河北株与国外肠艾美耳球虫(EF694012)18S rDNA相似性达99.9%.系统发育进化树显示,肠艾美耳球虫河北株与肠艾美耳球虫(EF694012)亲缘关系最近. |
| 关 键 词： | 肠艾美耳球虫 18S rDNA 测序 遗传进化树 |
The Sequence and Phylogenetic Analysis of 18S rDNA of E.intestinalis |
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| FANG Sufang,CUI Ping,GU Xiaolong and SUO Xun. The Sequence and Phylogenetic Analysis of 18S rDNA of E.intestinalis[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2011, 20(5): 31-34 | |
| Authors: | FANG Sufang CUI Ping GU Xiaolong SUO Xun |
| Affiliation: | FANG Sufang1,2,CUI Ping2,GU Xiaolong2 and SUO Xun1(1.Colloge of Veterinary Medicine,China Agricultural University,Beijing 100193,China,2.Colloge of Animal Science and Technology,Hebei North University,Zhang Jiakou Hebei 075000,China) |
| Abstract: | Using single-oocyst seperation technology,E.intestinalis was isolated from rabbit in Hebei,then inoculated coccidia-free rabbits to propagate.Its genomic DNA was extracted by the method of CATB.Using conservative primer of 18S rDNA of Eimeria,18S rDNA gene fragment of E.intestinalis HB was amplified and sequenced.Among E.intestinalis HB and 11 species of rabbit-infecting Eimeria in the GenBank,the phylogenetic tree was constructed based on their 18S rDNA sequences by DNAstar software.The amplification resul... |
| Keywords: | E.intestinalis 18S rDNA Sequence Phylogenetic tree |
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