| 中华山蓼克隆的分子鉴定 |
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| 引用本文： | 李芳,宋垚彬,陈琢,李钧敏.中华山蓼克隆的分子鉴定[J].西北林学院学报,2011,26(4):104-107. |
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| 作者姓名： | 李芳 宋垚彬 陈琢 李钧敏 |
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| 作者单位： | 1. 台州学院生命科学学院,浙江临海,317000
2. 中国科学院植物研究所植被与环境变化国家重点实验室,北京,100093 |
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| 摘 要： | 采用ISSR分子标记对21个中华山蓼克隆进行了鉴定,结果表明:从100个ISSR引物中筛选出8个引物,对21个中华山蓼克隆进行DNA扩增,共扩增出84个清晰条带,其中44条具多态性,平均多态比率为52.4%。共扩增出73种条带类型,其中特异性条带类型为40种。综合分析这些条带类型,可知只要通过8个引物中的3个即可将所有的克隆鉴定出来,表明ISSR技术在分子水平上鉴定中华山蓼的克隆是一种行之有效的方法。
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| 关 键 词： | 中华山蓼 克隆鉴定 ISSR |
Molecular Identification of Oxyria sinensis Clones |
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| LI Fang,SONG Yao-bin,CHEN Zhuo,LI Jun-min.Molecular Identification of Oxyria sinensis Clones[J].Journal of Northwest Forestry University,2011,26(4):104-107. |
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| Authors: | LI Fang SONG Yao-bin CHEN Zhuo LI Jun-min |
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| Institution: | LI Fang1,SONG Yao-bin2,CHEN Zhuo,LI Jun-min1(1.School of Life Sciences,Taizhou University,Linhai,Zhejiang 317000,China,2.Istitute of Botany Vegetation and State Key Laboratory of Environmental Change,Beijing 100093,China) |
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| Abstract: | Twenty one Oxyria sinensis clones were identified by means of ISSR molecular markers.The results showed that 84 distinguishable bands were amplified using 8 primers,which were selected from a total of 100 ISSR markers.Among them,44 bands were polymorphic and the percentage of polymorphic bands reached 52.4%.Seventy three kinds of band profiles were determined in 21 clones and 40 profiles were specific.Comprehensive analysis of these bands showed that 21 clones could be identified by only three primers: UBC ... |
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| Keywords: | Oxyria sinensis clone identification ISSR |
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