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| 水分胁迫对烤烟腺毛形态发育及基因表达的影响 | |
| 引用本文： | 张华,崔红,冀浩,梁志敏,翁梦苓. 水分胁迫对烤烟腺毛形态发育及基因表达的影响[J]. 郑州牧业工程高等专科学校学报, 2012, 32(1): 4-8 |
| 作者姓名： | 张华 崔红 冀浩 梁志敏 翁梦苓 |
| 作者单位： | 1. 河南农业大学,河南郑州450002／郑州牧业工程高等专科学校,河南郑州450011 2. 河南农业大学,河南郑州,450002 3. 广东中烟工业有限责任公司,广东广州,510310 4. 郑州科地有机肥料有限公司,河南郑州,450001 5. 广西壮族自治区甘蔗研究所,广西南宁,530007 |
| 摘 要： | 以盆栽烤烟K326为材料,研究了在不同灌水条件下,烟叶生长发育过程中腺毛腺头形态发育的变化,以及土壤水分对成熟期烟草腺毛基因表达的影响。采用荧光电子显微镜观察腺毛发现：水分胁迫使得腺头细胞正常的生长-成熟-分泌周期缩短。表达谱基因芯片杂交结果：共发现有效差异表达（ratio值≥2或≤0.5）的基因75个,其中上调差异基因47个,主要集中在抗性相关基因15个,碳代谢相关基因7个,功能未知基因10个;下调差异基因28个,主要集中在次生代谢相关基因6个,碳代谢相关基因4个,功能未知基因11个。 |
| 关 键 词： | 烤烟腺毛 水分胁迫 形态发育 基因表达 |
Effects of Drought Stress on the Development and Gene Expression of Tobacco Trichomes |
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| ZHANG Hua,CUI Hong,JI Hao,LIANG Zhi-min,WENG Meng-ling. Effects of Drought Stress on the Development and Gene Expression of Tobacco Trichomes[J]. Journal of Zhengzhou College of Animal Husbandry Engineering, 2012, 32(1): 4-8 | |
| Authors: | ZHANG Hua CUI Hong JI Hao LIANG Zhi-min WENG Meng-ling |
| Affiliation: | 1.Henan Agricultural University,Zhengzhou Henan 450002,China;2.ZhengZhou college of Animal Husbandry Engineering,Zhengzhou Henan 450011,China;3.China Tobacco Guangdong Industrial Co.,Ltd.,Guangzhou GuangDong 510310,China;4.Zhengzhou Code Orginc Fertilizer Co.,Ltd.,Zhengzhou Henan 450001,China;5.Guangxi Sugarcane Research Institute,Nanning Guangxi 530007,China) |
| Abstract: | Taking the flue-cured tobacco K326 potted as experiment materials,under the different irrigation conditions,we studied the morphology development of trichomes,the ultra-structure of the head cell of flue-cured tobacco trichome and the effect of soil water on related gene expression in mature stage.The observation by fluorescence microscopy indicated that:the drought stress shortened the growth-maturity-secretion cycle of trichome head cells.The cDNA micro-array analysis showed that:there were 75 differently expressed genes(with ratio values ≥2 or ≤0.5)in total.Amid them,47 appeared to be up-regulated among which maybe 15 genes were related to defense,7 genes were related to carbolic metabolism processes and 10 genes were function-unknown;other 28 appeared to be down-regulated among which,perhaps,6 genes were related to secondary metabolism,4 genes were related to carbolic metabolism processes and 11 genes were function-unknown. |
| Keywords: | flue-cured tobacco trichomes drought stress morphology development gene expression |
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