| 文心兰茎尖诱导原球茎的影响因子研究 |
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| 引用本文： | 张超,陈立思,王广东,郭维明,曾武清,杨斌. 文心兰茎尖诱导原球茎的影响因子研究[J]. 广东农业科学, 2008, 0(4): 27-31 |
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| 作者姓名： | 张超 陈立思 王广东 郭维明 曾武清 杨斌 |
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| 作者单位： | 广州花卉研究中心,广东,广州,510360;南京农业大学园艺学院,江苏,南京,210095;广州花卉研究中心,广东,广州,510360;南京农业大学园艺学院,江苏,南京,210095 |
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| 摘 要： | 以文心兰无菌幼苗茎尖为试材,研究了基本培养基、植物生长调节剂、碳源、培养基pH值及茎尖放置方式对原球茎诱导的影响。结果表明:1/2MS为原球茎诱导的适宜培养基,0.1 mg/L TDZ最适合原球茎诱导且无不定芽分化、诱导率达80%,30 g/L蔗糖是原球茎诱导的适宜碳源,培养基pH值为5.2时原球茎诱导效果最佳,茎尖竖直放置比水平放置更有利于原球茎诱导。
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| 关 键 词： | 文心兰 茎尖培养 原球茎 诱导 |
| 文章编号： | 1004-874X(2008)04-0027-05 |
| 修稿时间： | 2008-01-18 |
Study on some influential factors in protocorm-like bodies induction by shoot tips culture of Oncidium |
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| ZHANG Chao,CHEN Li-si,WANG Guang-dong,GUO Wei-ming,ZENG Wu-qing,YANG Bin. Study on some influential factors in protocorm-like bodies induction by shoot tips culture of Oncidium[J]. Guangdong Agricultural Sciences, 2008, 0(4): 27-31 |
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| Authors: | ZHANG Chao CHEN Li-si WANG Guang-dong GUO Wei-ming ZENG Wu-qing YANG Bin |
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| Abstract: | The effects of basic medium,plant growth regulators,carbon sources,medium pH and inoculation position on protocorm-like bodies(PLBs) induction were studied by shoot tips culture of Oncidium.The results showed that 1/2MS was the suitable basal medium for PLBs induction;0.1 mg/L TDZ was found to be more effective in the induction of PLBs without shoots induced and the induction rate of PLBs was 80%;sucrose was the suitable carbon source for PLBs induction at the concentration of 30 g/L;PLBs could be induced most efficiently in a medium with pH 5.2;culturing the shoot tips vertically could promote more PLBs formation than culturing them horizontally. |
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| Keywords: | Oncidium shoot tips culture protocorm-like bodies induction |
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