农杆菌介导的CBF1基因对松南结缕草的遗传转化

CBF1（C-repeat/dehydration-responsive element binding factors 1）基因为来源于拟南芥的一类与干旱、高盐及低温耐性有关的转录因子基因。松南结缕草（Zoysia sp.）是本实验室育成的形态特征介于日本结缕草与沟叶结缕草之间的一种中型结缕划，在长江以南地区广泛种植。

Agrobacterium-mediated CBF1 Gene Transformation of Songnan Zoysia Grass

AIM: To explore the effect and the corresponding mechanism of medium chain triglyceride (MCT) on CYP7A1 gene expression in mice. METHODS: C57BL/6J mice (15/group) were respectively received mash as AIN-93G formula (basic control BC), or 1% cholesterol supplemented AIN-93G formula (Chol), or 1% cholesterol and 14% long chain triglyceride (LCT) rich in myristic acid supplemented AIN-93G formula (Chol+LCT), or 1%cholesterol and 14% MCT (caprylic acid/capric acid: 3/1) supplemented AIN-93G formula (Chol+MCT) for 6 weeks. The change of serum total cholesterol (TC), the content of cholesterol in liver, the bile acid pool of mice and the expression of cholesterol 7-hydroxylase (CYP7A 1) gene were investigated. RESULTS: Compared to mice fed Chol diet, the mice fed Chol+MCT diet had the lower serum TC (P<0.01), higher liver cholesterol (P<0.01), smaller bile acid pool (P<0.01) and lower CYP7A1 gene expression (P<0.01). Compared to mice fed Chol+LCT diet, the mice of Chol+MCT group had lower serum TC (P<0.01), higher bile acid pool (P<0.01) and higher CYP7A1 gene expression (P<0.01). The levels of liver cholesterol were almost the same. CONCLUSION: The results of this study demonstrate that the effect of MCT on cholesterol level is related to CYP7A1 gene expression.