| 马铃薯抗逆基因Fe-SOD的克隆与序列分析 |
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| 引用本文： | 武新娟,魏峭嵘,石瑛,王凤义. 马铃薯抗逆基因Fe-SOD的克隆与序列分析[J]. 东北农业大学学报, 2009, 40(4) |
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| 作者姓名： | 武新娟 魏峭嵘 石瑛 王凤义 |
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| 作者单位： | 东北农业大学农学院,哈尔滨,150030 |
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| 摘 要： | 文章以马铃薯克新19号为材料,提取植株叶片总DNA,利用同源序列法,根据GenBank中已登陆番茄的Fe-SODmRNA序列,设计1对引物,经PCR扩增获得了大小为1413bp的片段。序列分析表明,预测其含有4个外显子和3个内含子,编码了145个氨基酸序列,分子质量为16.255ku,等电点为5.88。其编码的氨基酸序列包含了两个Fe-SOD蛋白的保守结构域,与NCBI数据库中其他植物的Fe-SOD蛋白同源性很高,与番茄中58～203相关片段同源性高达93.79%。该基因已在GenBank上注册,登录号为EU545469。
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| 关 键 词： | 马铃薯 Fe-SOD 克隆 序列分析 |
Cloning and sequence analysis of Fe-SOD from potato |
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| WU Xinjuan,WEI Qiaorong,SHI Ying,WANG Fengyi. Cloning and sequence analysis of Fe-SOD from potato[J]. Journal of Northeast Agricultural University, 2009, 40(4) |
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| Authors: | WU Xinjuan WEI Qiaorong SHI Ying WANG Fengyi |
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| Affiliation: | College of Agriculture;Northeast Agricultural University;Harbin 150030;China |
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| Abstract: | In this experiment,the total DNA was extracted from the variety Kexin No.19,and a pair of primers was designed according to the conserved regions of the Fe-SOD mRNA from tomato in GenBank.A 1 413 bp fragment was got by PCR amplication.The results of sequence analysis showed that the sequence contained four extrons and three introns,and encoded 145 amino acids.The molecular weight was 16.255 ku,and its isoelectric was 5.88.This sequence had two domains encoding Fe-SOD protein.The sequence with Fe-SOD of othe... |
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| Keywords: | Fe-SOD |
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