| 陕西省本氏针茅自然种群遗传多样性的ISSR分析 |
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| 引用本文： | 俞靓,井赵斌,程积民. 陕西省本氏针茅自然种群遗传多样性的ISSR分析[J]. 草地学报, 2012, 20(3): 512-517. DOI: 10.11733/j.issn.1007-0435.2012.03.020 |
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| 作者姓名： | 俞靓 井赵斌 程积民 |
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| 作者单位： | 1.西北农林科技大学资源环境学院,陕西杨凌,712100;2.西北农林科技大学动物科技学院,陕西杨凌,712100;3.西北农林科技大学资源环境学院,陕西杨凌712100;西北农林科技大学动物科技学院,陕西杨凌712100;中国科学院水利部水土保持研究所,陕西杨凌712100 |
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| 基金项目： | 国家现代农业产业技术体系建设—成阳牧草试验站项目,中国科学院院地合作专项 |
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| 摘 要： | 利用ISSR分子标记,对采自陕西省不同地区的5个本氏针茅(Stipa bungeana)自然种群进行遗传变异及群体遗传结构分析,旨在探讨本氏针茅遗传变异产生的分子生态机理,为其资源保护提供理论依据.在100个供试单株中,采用15条ISSR引物共扩增出223条可统计条带,其中多态性带182条,多态性位点比率为81.61％,Nei's基因多样性(H)为0.1887,Shannon信息指数(I)为0.2975,各种群之间存在较高的多态性.种群间的遗传变异为63.01％,种群内的遗传变异为36.99％,遗传分化系数Φn为0.6300,种群间的基因流(Nm)为0.1468,表明本氏针茅遗传分化程度较高,
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| 关 键 词： | 本氏针茅 ISSR 遗传多样性 遗传结构 |
| 收稿时间： | 2011-11-10 |
Genetic Diversity of Stipa bungeana Trin.Populations in Shaanxi Analyzed by ISSR Markers |
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| YU Jing , JING Zhao-bin , CHENG Ji-min. Genetic Diversity of Stipa bungeana Trin.Populations in Shaanxi Analyzed by ISSR Markers[J]. Acta Agrestia Sinica, 2012, 20(3): 512-517. DOI: 10.11733/j.issn.1007-0435.2012.03.020 |
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| Authors: | YU Jing JING Zhao-bin CHENG Ji-min |
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| Affiliation: | 1. College of Resources and Environment, Northwest A&F University, Yangling, Shaanxi Province 712100, China;2. College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi Province 712100, China;3. Institute of Soil and Water Conservation, Chinese Academy of Sciences and Ministry of Water Resources, Yangling, Shaanxi Province 712100, China |
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| Abstract: | Inter-simple sequence repeat(ISSR) markers were used to determine the genetic diversity and genetic differentiation of five Stipa bungeana natural populations in Shaanxi province.Objectives of this study were to explore the molecular and ecology mechanisms of genetic diversity of Stipa bungeana.Fifteen primers screened from 96 primers amplified stable bands.Among a total of 223 amplified bands,182(81.61%) bands were polymorphic loci.Nei’s gene diversity index and Shannon’s information index were 0.1887 and 0.2975,respectively,which indicated high genetic diversity existing among population.Analysis of molecular variance(AMOVA) demonstrated genetic variation mainly among populations.Genetic variation rate was 63.01% among populations,but 36.99% within population.Moreover,the level of gene flow(Nm) was measured to be 0.1139 individual per generation among populations that indicated Stipa bungeana had a higher genetic differentiation. |
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| Keywords: | Stipa bungeana Trin. ISSR Genetic diversity Genetic structure |
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