| 以无菌苗外植体高效诱导球茎甘蓝不定芽再生研究 |
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| 引用本文： | 马光,郭继平.以无菌苗外植体高效诱导球茎甘蓝不定芽再生研究[J].北方园艺,2009(9). |
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| 作者姓名： | 马光 郭继平 |
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| 作者单位： | 衡水学院,生命科学系,河北,衡水,053000;东北林业大学,生命科学学院,黑龙江,哈尔滨,150040;衡水学院,生命科学系,河北,衡水,053000;哈尔滨工业大学,食品科学与工程学院,黑龙江,哈尔滨,150090 |
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| 摘 要： | 以球茎甘蓝(Brassica oleracea L. var. caulorapa DC.) 5 d苗龄的无菌苗带柄子叶和胚轴为外植体,研究了不同激素组合对其不定芽再生的影响.结果表明:TDZ同NAA配合诱导再生的效果好于6-BA同NAA的组合;带柄子叶再生效果好于胚轴.最佳再生培养基为添加3.5 mg/L TDZ+0.5 mg/L NAA的含5.0 mg/L AgNO3的MS培养基.
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| 关 键 词： | 球茎甘蓝 再生 TDZ 带柄子叶 胚轴 |
Efficient Shoot Regeneration of Brassica caulorapa Using Seed Explants Cultured in Vitro |
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| MA Guang,GOU Ji-ping.Efficient Shoot Regeneration of Brassica caulorapa Using Seed Explants Cultured in Vitro[J].Northern Horticulture,2009(9). |
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| Authors: | MA Guang GOU Ji-ping |
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| Institution: | 1.Department of Life Sciences;Hengshui College;Hengshui;Hebei 053000;China;2.College of Life Sciences;Northeast Forestry University;Harbin;Heilongjiang 150040;3.College of Food Science and Engineering;Harbin Institute of Technology;Heilongjiang 150090;China |
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| Abstract: | The shoot regeneration of kohlrabi(Brassica oleracea L.var.caulorapa DC.)in vitro from cotyledonary petiole and hypocotyl explants was studied.The effects of the combination and concentration of plant growth regulators were investigated on shoot regeneration of kohlrabi.The results showed that combination of thidiazuron(TDZ) and NAA was better than combination of 6-BA and NAA and cotyledonary petiole was better than hypocotyl for regeneration.The most suitable recipe for getting high shoot regeneration was ... |
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| Keywords: | TDZ |
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