Characterization of isozymes in Salvia columbariae |
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Authors: | Ahmad Hashemi Ali Estilai Jirair Carapetian |
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Institution: | (1) Department of Botany and Plant Sciences, University of California, 92521-0124 Riverside, CA, USA |
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Abstract: | Summary
Salvia columbariae is a herbaceous annual species which has potential for domestication as a new source of industrial oil. Isozyme markers provide a mean by which this process may be facilitated. Isozyme survey of field grown Salvia columbariae plants showed variation for malate dehydrogenase (MDH), phosphoglucose isomerase (PGI), and phosphogllucomutase (PGM). Selfed seed was obtained from the field and was grown in the greenhouse for segregation analyses. Electrophoretic results indicated that MDH was variable at zone 1, showing presence or absence of a band. The observed segregation ratio was not significantly different from expected ratio for Pgi-4 and Pgm-3 isozymes, indicating monogenic control of the two loci. Pgi-4 locus was heterozygous for a null allele. Cross dimerization between the allozyme Pgi-3 and Pgi-4 loci resulted in an intergenic band for this isozyme system.Abbreviations ACO
aconitase
- ADH
alcohol dehydrogenase
- DTT-DL
dithiothreitol
- FDH
formate dehydrogenase
- GOT
glutamate-oxalacetate transaminase
- IDH
isocitric dehydrogenase
- MDH
malate dehydrogenase
- MNR
menadione reductase
- 6PGD
6-phosphogluconate dehydrogenase
- PGI
phosphoglucose isomerase
- PGM
phosphoglucomutase
- PVP-40
polyvinylpyrrolidone
- SKDH
shikimate dehydrogenase
- TPI
triosephosphate isomerase |
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Keywords: | chia isozymes oilseed Salvia columbariae |
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