Recovery of Trichinella Spiralis Larvae From Frozen Muscle Samples |
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Authors: | Sv. Aa. Henriksen |
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Affiliation: | The State Veterinary Serum Laboratory, Bulowsvej 27 DK-1870 Copenhagen V, Denmark |
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Abstract: | Demonstration of Trichinella spiralis in suspensions of digested muscle tissue has until now mainly been based on methods utilizing the coiling and/or the motility of the living larvae. Considering this, Jackson (1977) estimated that, compared to the number observed by examination of corresponding unfrozen samples, 78 % of larvae in frozen samples would be lost, when an ordinary pepsin-HCl digestion technique was used. |
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