Enhancement of biocontrol efficacy of Pichia membranaefaciens by hot water treatment in postharvest diseases of citrus fruit |
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| Affiliation: | 1. College of Food Science, Southwest University, Chongqing 400715, PR China;2. Laboratory of Quality & Safety Risk Assessment for Agro-products on Storage and Preservation (Chongqing), Ministry of Agriculture, Chongqing 400715, PR China;1. Postharvest Technology Research Laboratory, South African Research Chair in Postharvest Technology, Department of Food Science, Faculty of AgriSciences, Stellenbosch University, Private Bag X1, Stellenbosch 7602, South Africa;2. Department of Horticultural Engineering, Leibniz-Institute for Agricultural Engineering and Bioeconomy (ATB), Potsdam-Bornim, D-14469 Potsdam, Germany;3. Postharvest Technology Research Laboratory, South African Research Chair in Postharvest Technology, Department of Horticultural Science, Faculty of AgriSciences, Stellenbosch University, Private Bag X1, Stellenbosch 7602, South Africa;1. Department of Agricultural Education, Faculty of Industrial Education and Technology, King Mongkut’s Institute of Technology Ladkrabang, Ladkrabang, Bangkok, Thailand;2. Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu City, Gifu 501-1193, Japan;3. The United Graduate School of Agricultural Science, Gifu University, 1-1 Yanagido, Gifu City, Gifu, 501-1193, Japan;1. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, 2000 Rosario, Argentina;2. Estación Experimental Concordia, Instituto Nacional de Tecnología Agropecuaria (INTA), Colonia Yeruá, Argentina |
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| Abstract: | Effects of hot water treatment (HWT; 53 °C for 2 min) and Pichia membranaefaciens, either alone or in combination, on controlling Penicillium italicum, Penicillium digitatum, and natural infection in citrus fruit were investigated. Results showed that the combined treatment significantly reduced disease incidence and lesion diameters of blue and green molds in artificially inoculated fruit whether the pathogens were inoculated after or before the treatment compared with the treatment of P. membranaefaciens or HWT alone. The combination of P. membranaefaciens with HWT was as effective as the fungicide treatment in natural infection trials. Application of HWT did not affect the growth of P. membranaefaciens in the wounds of citrus fruit at 20 °C or 4 °C. P. membranaefaciens combined with HWT effectively enhanced the phenylalanine ammonia-lyase, peroxidase, polyphenoloxidase, chitinase, and β-1,3-glucanase activities and stimulated the synthesis of phenolic compounds. These results suggest that the use of HWT is a useful approach to improve the efficacy of P. membranaefaciens in postharvest diseases of citrus fruit. |
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| Keywords: | Hot water treatment Biological control Citrus fruit HWT" },{" #name" :" keyword" ," $" :{" id" :" kwrd0045" }," $$" :[{" #name" :" text" ," _" :" Hot Water Treatment CFU" },{" #name" :" keyword" ," $" :{" id" :" kwrd0055" }," $$" :[{" #name" :" text" ," _" :" Colony-forming Unit NYDA" },{" #name" :" keyword" ," $" :{" id" :" kwrd0065" }," $$" :[{" #name" :" text" ," _" :" Nutrient Yeast Dextrose Agar NYDB" },{" #name" :" keyword" ," $" :{" id" :" kwrd0075" }," $$" :[{" #name" :" text" ," _" :" Nutrient Yeast Dextrose Broth POD" },{" #name" :" keyword" ," $" :{" id" :" kwrd0085" }," $$" :[{" #name" :" text" ," _" :" Peroxidase PPO" },{" #name" :" keyword" ," $" :{" id" :" kwrd0095" }," $$" :[{" #name" :" text" ," _" :" Polyphenoloxidase PAL" },{" #name" :" keyword" ," $" :{" id" :" kwrd0105" }," $$" :[{" #name" :" text" ," _" :" Phenylalanine Ammonia-lyase CHI" },{" #name" :" keyword" ," $" :{" id" :" kwrd0115" }," $$" :[{" #name" :" text" ," _" :" Chitinase |
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