严重急性呼吸道综合征(SARS)病毒基因组比较分析与进化研究

“严重急性呼吸道综合征”(SARS)由于危害严重，引起人们的高度重视，在各国科研人员努力下，目前已完成了十几个毒株的分离与全基因组测序工作。本研究对世界各地分离的SARS毒株的基因组全序列进行比较，发现不同毒株的同源性在99．8％以上，SARS病毒的遗传稳定性较高；将SARS病毒与其它冠状病毒基因组以及编码蛋白质序列进行比较分析，证实SARS病毒是一种新的冠状病毒，可能进化起源较早，并发现SARS病毒和牛冠状病毒同源程度最高。SARS病毒orflab、orfla、S、M、N编码区氨基酸序列和牛冠状病毒同源程度最高，鼠肝炎病毒次之。

Comparative Analysis and Evolution Studies on Severe Acute Respiratory Syndrome Genome

Severe acute respiratory syndrome (SARS) was a recently described disease of humans that had spread widely over the past 6 months. To reveal the genome organization and phylogeny of SARS virus (SARS-CoV), 13 complete sequences of SARS CoV genome and 6 complete sequences of other coronavirus genome from GenBank were compared. Multiple alignment indicated that the identities of the different SARS-CoV strains were more than 98. 5%,which suggests the same evolutional origin. However, SARS-CoV seems experiencing possible slowly genetic variation. Phylogenetic analysis of the predicted viral proteins showed that SARS-CoV does not closely resemble any of the three previously known groups of coronaviruses, indicating that SARS-CoV is a new coronavirus.