柑桔炭疽菌果胶裂解酶pelB基因特异性引物的筛选

以柑桔炭疽菌为主要研究对象,根据已知的炭疽菌果胶裂解酶pelB基因序列,运用GenBank及Primer Premier 5共设计6对引物,其中编号为PB1/PB2、Cg1A/Cg1b及Cg2a/Cg2b的引物可扩增出pelB基因片段,经同源性分析,全序列与GenBank中登录号为GU478342.1 pelB基因的的同源性高达98%,PB1/PB2、Cg1A/Cg1b对柑桔胶孢炭疽菌果胶裂解酶pelB基因有较高特异性。

Screening of pelB-specific primers for pectate lyase gene of Colletotrichum gloeosprioides

Colletotrichum gloeosprioides was the main object in this study.Six pairs of primers according to the conservative sequence of the reported pelB gene were designed with GenBank and Primer Premier 5 and then the six pairs of primers were amplified by PCR.The result showed that the primers numbered PB1/PB2,Cg1A/Cg1b and Cg2a/Cg2b could be amplified pelB gene fragment.Comparing and analysising the sequencing result to the reported sequence showed that the amplified fragment of pelB gene was above 98% homology with GenBank the accessioned number GU478342.1.However,the three specific designed primers were not specific with other common pathogens.