SLE患者CD4+CD25+调节性T细胞及Foxp3基因表达的研究

目的:研究系统性红斑狼疮(SLE)患者外周血CD 4 CD 25 调节性T细胞及Foxp3基因的表达水平,了解它们在SLE发病机制中的作用。方法:分别收集25例SLE患者(SLE组)及健康人(对照组)外周抗凝静脉血,分离纯化T淋巴细胞。PE标记抗CD 4单抗,F ITC标记的抗CD 25单抗,作双色流式细胞术,分析SLE患者外周血CD 4 CD 25 调节性T细胞百分率,RT-PCR检测T细胞Foxp3 mRNA表达。结果:SLE组外周血CD 4 T、CD 4 CD 25 T细胞百分率及T细胞Foxp3 mRNA水平均低于对照组(P<0.01),并且CD 4 CD 25 T细胞百分率与Foxp3mRNA水平呈依赖关系(P<0.01)。结论:SLE患者外周血存在细胞免疫功能失调,CD 4 CD 25 调节性T细胞数量减少和Foxp3mRNA表达下调可能与SLE的免疫学发病机制有关。

Expression of CD4+CD25+ regulatory T cell and Foxp3 gene in systemic lupus erythematosus

Objective: To observe the expression of CD4~+CD25~+ regulatory T cell and Foxp3 gene from peripheral blood in patients with systemic lupus erythematosus(SLE),and to investigate their roles in the pathogenesis of SLE.Methods: The percentage of CD4~+CD25~+ regulatory T cells was detected by bicolor flow cytometry,and the expression of Foxp3 mRNA on T lymphocytes was determined by RT-PCR in 25 SLE patients and 25 healthy controls.Results: The percentage of CD4~+ cells and CD4~+CD25~+ T cells,and level of Foxp3 mRNA on T lymphocytes were significantly lower in SLE group than those in control group(P<0.01).There was a positive correlation between the percentage of CD4~+CD25~+ T cell expression and expression of Foxp3 mRNA(r=0.786,P<0.01) in SLE group.Conclusion:There is a disturbance of cellular immune function in SLE.The downexpression of CD4~+CD25~+ regulatory T cell and Foxp3 mRNA on T lymphocytes may be involved in the immunopathogenesis of SLE.