| 牛瑟氏泰勒虫吉林株ITS基因的克隆与系统进化分析 |
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| 引用本文： | 钱年超,张影,薛书江,贾立军,张守发.牛瑟氏泰勒虫吉林株ITS基因的克隆与系统进化分析[J].畜牧与兽医,2011,43(6). |
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| 作者姓名： | 钱年超 张影 薛书江 贾立军 张守发 |
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| 作者单位： | 延边大学农学院动物医学系,吉林延吉,133002 |
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| 摘 要： | 本研究根据GenBank上的牛瑟氏泰勒虫内转录间隔区基因(ITS基因)设计合成1对特异性引物,以采自吉林省珲春市某养牛场的血液样本为试验材料,PCR扩增牛瑟氏泰勒虫ITS基因,克隆至pMD-18T Simple载体,进行序列测定,将该基因序列与GenBank上9种已知的泰勒虫相应序列进行比较分析,建立系统进化树。结果表明:牛瑟氏泰勒虫吉林株与美国株亲缘关系较近,其次是水牛泰勒虫,与其他泰勒虫亲缘关系较远。
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| 关 键 词： | 牛瑟氏泰勒虫 ITS基因 克隆 系统发育树 |
Cloning and phylogenetic analysis of ITS gene from Theileria sergenti Jilin strain |
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| QIAN Nian-chao,ZHANG Ying,XUE Shu-jiang,JIA Li-jun,ZHANG Shou-fa.Cloning and phylogenetic analysis of ITS gene from Theileria sergenti Jilin strain[J].Animal Husbandry & Veterinary Medicine,2011,43(6). |
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| Authors: | QIAN Nian-chao ZHANG Ying XUE Shu-jiang JIA Li-jun ZHANG Shou-fa |
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| Institution: | QIAN Nian-chao,ZHANG Ying,XUE Shu-jiang,JIA Li-jun,ZHANG Shou-fa (Department of Veterinary Medicine,Agriculture College of Yanbian University,Yanji 133002,China) |
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| Abstract: | A pair of specific primers was synthesized according to Theileria sergenti internal transcribed spacers(ITS) gene in GenBank.Blood samples were collected from a cattle farm in Hunchun,Jilin province.T.sergenti ITS gene was amplified by PCR and cloned into pMD-18T Simple vector for sequencing.The result of sequence analysis was compared with those of 9 other Theileria strains available in GenBank and the phylogenetic tree was constructed.The result showed that T.sergenti Jilin strain is closer to American st... |
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| Keywords: | Theileria sergenti ITS gene clone phylogenetic tree |
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