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A Rapid DNA Mini-prep Method for Large-Scale Rice Mutant Screening |
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| Authors: | QIU Fu-lin WANG He-he CHEN Jie ZHUANG Jie-yun Hei LEUNG CHENG Shi-hua Wu Jian-li |
| Affiliation: | 1.Shenyang Agricultural University, Shenyang 110101, China;2. Chinese National Center for Rice Improvement/State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China;3. Rice Research Institute, Liaoning Academy of Agricultural Sciences, Shenyang 110101, China;4. Entomology and Plant Pathology Division, International Rice Research Institute, DAPO P. O. Box 7777, Metro Manila, Philippines |
| Abstract: | A high throughput rice DNA mini-preparation method was developed. The method is suitable for large-scale mutant bank screening as well as large mapping populations with characteristics of maintaining relatively high level of DNA purity and concentration. The extracted DNA was tested and suitable for regular PCR amplification (SSR) and for Targeting Induced Local Lesion in Genome (TILLING) analysis. |
| Keywords: | rice targeting induced local lesion in genome DNA extraction polymerase chain reaction |
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