Effect of MON-0585 on sclerotization of Aedes aegypti cuticle |
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| Affiliation: | 1. Al-Hussein Teaching Hospital, Directorate of Al-Muthanna Health, Ministry of Health, Iraq;2. Directorate of Education Al-Muthanna, Ministry of Education, Iraq;3. Iraqi Cement State Company, Ministry of Industry and Minerals, Baghdad, Iraq;4. Department of Physics and Chemistry, Faculty of Applied Sciences and Technology, University Tun Hussain Onn Malaysia, Pagoh Campus, Jalan Pancor, 84600 Pancor, Johor, Malaysia;1. University of Bremen, Center for Environmental Research and Technology, General and Theoretical Ecology, Leobener Str., 28359 Bremen, Germany;2. Technical University of Denmark (DTU), Department of Environmental Engineering, Miljoevej 113, 2800 Kgs., Lyngby, Denmark;1. Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, PR China;2. University of Chinese Academy of Sciences, Beijing 100049, PR China;1. School of Chemistry, University of Leeds, Woodhouse Lane, Leeds LS2 9JT, UK;2. CNRS, University of Bordeaux, ICMCB, UPR 9048, F-33600 Pessac, France;1. Escola Politécnica, Universidade Federal da Bahia, Rua Aristides Novis, 2, 6° andar, Federação, 40210-630, Salvador, Bahia, Brazil;2. Departamento de Farmácia, Universidade Federal da Bahia, Rua Barão de Jeremoabo, s/n, Ondina, 40170-115, Salvador, Bahia, Brazil;3. CIEPQPF, Department of Chemical Engineering, FCTUC, University of Coimbra, Rua Sílvio Lima, Pólo II—Pinhal de Marrocos, 3030-790 Coimbra, Portugal |
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| Abstract: | The effect of MON-0585, an insecticide affecting sclerotization, on mosquito cuticle was investigated. Since the lethal effect of MON-0585 is elicited during cuticle development, cuticular compoments such as structural proteins, chitin, phenoloxidase, and phenols were analyzed from both control and insecticide-treated larvae. Soluble cuticular proteins isolated from both groups of animals did not show any difference in electrophoretic mobility nor in amino acid composition. Cuticle bound proteins also failed to show any differences in amino acid composition. Transmission electron microscopic and radioactive tracer studies indicated that neither chitin synthesis nor chitin fiber assembly in cuticle was affected by MON-0585 treatment. Significant reductions in the levels of covalently bound catechols were observed in MON-0585-treated animals. Moreover, these animals exhibited reduced phenoloxidase activity as compared to controls. Scanning electron microscopic studies revealed malformations in the exoskeleton and in the breathing tubes of poisoned mosquito pupae. These results indicate that MON-0585 interferes with cuticular stabilization indirectly and disrupts the structure of the breathing tubes causing early pupal death. |
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