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DNA序列分析在植物分子系统学研究中的应用现状——-以蔷薇科为例
引用本文:郑小艳,曹家树,滕元文. DNA序列分析在植物分子系统学研究中的应用现状——-以蔷薇科为例[J]. 园艺学报, 2009, 36(12): 1827-1836
作者姓名:郑小艳  曹家树  滕元文
作者单位:(浙江大学园艺系, 农业部园艺植物生长发育与品质调控重点开放实验室, 杭州310029)
基金项目:国家自然科学基金,浙江省自然科学基金杰出青年团队项目,中国博士后科学基金 
摘    要: 近20年来, DNA序列已被广泛应用于植物各分类阶元的系统学研究中, 为解决长期有争议的和亟待解决的系统进化问题提供了有力的证据。现以蔷薇科为例, 概述了应用DNA片段进行植物分子系统研究的现状, 详细剖析了应用DNA序列进行植物系统发育研究时常见的问题及其原因, 提出了对存在多倍化、杂交起源和快速分化等复杂进化史的植物类群进行系统学分析时选用DNA序列的策略和注意事项。

关 键 词:蔷薇科  DNA序列  系统发育  ITS假基因  低拷贝核基因
收稿时间:2009-06-01
修稿时间:2009-11-19

Research Progress of Plant Molecular Phylogenetic Analyses Based on DNA Sequence Data-A Case Review in Rosaceae
ZHENG Xiao-yan,CAO Jia-Shu,TENG Yuan-wen. Research Progress of Plant Molecular Phylogenetic Analyses Based on DNA Sequence Data-A Case Review in Rosaceae[J]. Acta Horticulturae Sinica, 2009, 36(12): 1827-1836
Authors:ZHENG Xiao-yan  CAO Jia-Shu  TENG Yuan-wen
Affiliation:(Department of Horticulture, the State Agricultural Ministry Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Hangzhou 310029, China)
Abstract:During the last twenty years,DNA sequences have been widely used in phylogenetic analyses of plants at the different taxonomic levels,and provided convincing evidence to solve the long-standing disputed or unsolved phylogenetic problems.In this paper,the current status of applications of DNA fragments in plant phylogenetic studies were briefly reviewed taking Rosaceae as an example,and the common troubles occurring in phylogenetic studies and the possible reasons were discussed in detail.Finally,the strategies and points for attention in selecting DNA sequences were proposed for phylogenetic reconstruction of plant taxa especially for plants that have undergone complex evolutionary processes such as hybridization,polyploidization and rapid radiation.
Keywords:Rosaceae  DNA sequence  phylogeny  ITS pseudogene  Low-copy nuclear gene
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