| 木聚糖降解细菌的鉴定及其木聚糖酶的特性 |
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| 引用本文： | 陈春岚,冯家勋.木聚糖降解细菌的鉴定及其木聚糖酶的特性[J].安徽农业科学,2009,37(13):5857-5860. |
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| 作者姓名： | 陈春岚 冯家勋 |
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| 作者单位： | 广西大学生命科学与技术学院,广西南宁,530005;广西亚热带生物资源保护利用重点实验室,广西南宁,530005;贺州学院,广西贺州,542800;广西大学生命科学与技术学院,广西南宁,530005;广西亚热带生物资源保护利用重点实验室,广西南宁,530005 |
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| 基金项目： | 国家高技术研究发展计划(863计划)项目(2007AA021307); 广西科技攻关计划(0630003-7) |
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| 摘 要： | 目的]探讨木聚糖酶的生理生化特性。方法]采用平板活性筛选法从不同环境样品中筛选出木聚糖降解能力最强的菌株进行鉴定并对其所产粗木聚糖酶的特性进行研究。结果]菌株GXM1被鉴定为洋葱假单胞菌，菌株GXM4为类芽孢杆菌。菌株GXMI所产木聚糖酶的最适pH为6．0，最适温度为55℃，菌株GⅫ咐所产木聚糖酶的最适pH为6．0，最适温度为50℃。2株菌株所产的木聚糖酶在40℃以下较为稳定。Mn^2＋、Ca^2＋和Zn^2＋对菌株GXM1所产木聚糖酶有显著的激活作用，而Cu^2＋、Fe^2＋和Fe^2＋对其有显著的抑制作用；Ca^2＋和Fe^3＋对菌株GXM4所产木聚糖酶有显著的激活作用，而Mn^2＋、Zn^2＋和Fe^2＋对其有显著的抑制作用。结论]为高效木聚糖酶的生产应用奠定了理论基础。
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| 关 键 词： | 木聚糖降解细菌 鉴定 木聚糖酶 |
Identification of Bacterium Degrading Xylan and the Characteristics of Its Xylanase |
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| CHEN Chun-lan et al.Identification of Bacterium Degrading Xylan and the Characteristics of Its Xylanase[J].Journal of Anhui Agricultural Sciences,2009,37(13):5857-5860. |
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| Authors: | CHEN Chun-lan |
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| Institution: | CHEN Chun-lan et al (College of Life Science , Technology,Guangxi University,Nanning,Guangxi 530005) |
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| Abstract: | Objective] The aim was to discuss the physiological and biochemical characteristics of xylanase.Method] The strains with strongest xylan degradation ability were screened from samples in different environment by plate activity screening method and then identified.The characteristics of the crude xylanase produced by the screened strains were studied.Result] The strain GXM1 was identified as Burkholderia cepacia and the strain GXM4 was identified as Paenibacillus favisporus.The suitable pH value of xylana... |
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| Keywords: | Bacterium degrading xylan Identification Xylanase |
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