水稻巨大胚基因的分子定位

 应用粳稻金南风巨大胚突变体和籼稻南京11杂交F₂群体58个植株构建了包含74个分子标记位点的遗传连锁图。 对巨大胚基因进行了分子定位, 结果发现巨大胚基因与第7染色体上RG678紧密连锁,两者之间的遗传距离为6.2 cM,与同一染色体上的另一探针RZ395之间的遗传距离为13 cM。根据巨大胚(ge)、褐色果皮(Rc)基因经典连锁图上的位置,将第7染色体分子图谱与经典图谱进行了整合

The RFLP of Tagging of Giant Embryo Gene

Using 58 plants derived from the F2 population of the cross Nanjing 11 (indica) /Kinmaze ge mutant (japonica), a rice molecular linkage map containing 74 markers and referring to 12 linkage groups (chromosomes) was constructed. In the map, the ge gene was mapped on chromosome 7, linking tightly to RFLP markers RG678 and RZ395, with estimated distance of 6. 2 cM and 13 cM.According to the locations of ge and Re gene in classical and molecular linkage map, two kinds of linkage map of rice chromosome 7 were corresponded to each other.