| 马尾藻类多糖对猪卵母细胞体外成熟及孤雌激活的影响 |
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| 引用本文： | 文国艺,何若刚,谢炳坤,陈自洪,石德顺,王士长.马尾藻类多糖对猪卵母细胞体外成熟及孤雌激活的影响[J].中国兽医学报,2005,25(1):94-95. |
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| 作者姓名： | 文国艺 何若刚 谢炳坤 陈自洪 石德顺 王士长 |
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| 作者单位： | 广西大学,动物科技学院,广西,南宁,530005 |
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| 基金项目： | 广西自然科学基金资助项目 (9817114 ) |
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| 摘 要： | 就马尾藻类多糖对猪卵母细胞体外成熟及孤雌激活的影响进行了探索。试验1证明。体外培养时。成熟液添加马尾藻类多糖能促进卵丘细胞扩展，成熟培养47-48h，添加20g／L马尾藻类多糖能提高核成熟率(41．8％)，与对照组29.3％的核成熟率相比，二者差异显著；试验2证明，进行化学联合激活时，添加20g／L马尾藻类多糖(未添加LH)W获得60.4％孤雌激活分裂率。显著高于对照组42．6％的孤雌激活分裂率。
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| 关 键 词： | 卵母细胞体外成熟 对照组 影响 成熟率 联合 体外培养 LH 孤雌激活 马尾藻 添加 |
| 文章编号： | 1005-4545(2005)01-0094-02 |
| 修稿时间： | 2003年9月26日 |
The Relation of Sargassum Polysaccharide with In Vitro Maturation and Parthenogenesis of Porcine Oocyte |
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| WEN Guo-yi,HE Ruo-gang,XIE Bing-kun,CHEN Zi-hong,SHI De-shun,WANG Shi-chang.The Relation of Sargassum Polysaccharide with In Vitro Maturation and Parthenogenesis of Porcine Oocyte[J].Chinese Journal of Veterinary Science,2005,25(1):94-95. |
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| Authors: | WEN Guo-yi HE Ruo-gang XIE Bing-kun CHEN Zi-hong SHI De-shun WANG Shi-chang |
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| Abstract: | This research was carried out to study the effect of sargassum polysaccharide on in vitro maturation and parthenogenesis of porcine oocyte:sargassum polysaccharide in mature medium benefits the extension of cumulus cells,when porcine oocyte cultured in vitro for 47-48 h,20 mg/mL sargassum polysaccharide results in 41.8% polar body rate,comparing with control group(29.3%),the difference is significant.It also lead 60.4% parthenogenesis cleavage rate which is significant higher than that of control group(42.6%). |
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| Keywords: | porcine oocyte sargassum polysaccharide in vitro maturation parthenogenesis cleavage rate |
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