17β‐Estradiol,Progesterone and Testosterone Concentrations in Cystic Fluids and Response to GnRH Treatment after Emptying of Ovarian Cysts in Dairy Cows

The aim of this study was to determine possible links between steroidogenic activity of single ovarian cysts and response to intramuscular treatment with 20 μg of buserelin (GnRH‐analogue) after cyst emptying, in pluriparous Friesian cows bearing a singleton cyst treated not earlier than 55 days post‐partum. Progesterone, 17β‐estradiol and testosterone were determined in cystic fluids collected by needle aspiration of the cyst. Of the cows, 75.6% began ovarian cyclicity within 30 days after treatment with a conception rate of 64.7%. In this study it was found that as progesterone concentration in cystic fluids rose, the number of positive responses to the treatment fell.