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Mechanism of inhibitory effect of allophane and montmorillonite on some enzymes
Authors:Yoshiyuki Kobayashi  Shigenori Aomine
Institution:1. Faculty of Agriculture , Kyushu University , Fukuoka , Japan;2. National Institute of Animal Industry , Chiba;3. Faculty of Agriculture , Kyushu University , Fukuoka , Japan
Abstract:It is well established that the presence of clay in an enzyme-substrate system may reduce the activity of enzymes (1-3,5,6,8,11-14). This inhibition by the clay would be principally caused by the adsorption of enzyme and/or substrate on the clay particle. In the previous papers (1–3), the activity of some enzymes was found to be inhibited in various degrees by clays. The enzyme appears to be adsorbed on the clay particle in various ways according to the nature of adsorbate as well as adsorbent. This suggests that the enzyme is adsorbed by the clay so as not to combine with the substrate, or that the adsorbed enzyme molecule has a configuration different from that of a free enzyme molecule, reducing the activity of the enzyme.
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