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A new method for measuring microbial biomass nitrogen in soils: Direct extraction after toluene treatment
Authors:Shinjiro Kanazawa  Takashi Kunito
Affiliation:1. Department of Environmental Science and Technology , Faculty of Agriculture, Kagoshima University , Kagoshima , 890 , Japan;2. Department of Environmental Science and Technology , Faculty of Agriculture, Kagoshima University , Kagoshima , 890 , Japan;3. Faculty of Agriculture , The University of Tokyo , Bunkyo-ku, Tokyo , 113 , Japan
Abstract:Abstract

Phenolic compounds occurring naturally are reducing agents which react with hydrous Fe oxide and Mn oxides (Lehmann et al. 1987). Diphenols such as hydroquinone, resorcinol, and catechol were oxidatively darkened by the presence of hydrous Fe oxide though the degree of darkening was much less pronounced than that by Mn oxides (Shindo and Huang 1984). Okazaki et al. (1976) suggested that polyphenols are one of the materials responsible for the dissolution of Mn in a paddy soil under reducing conditions.
Keywords:K N nin factor  microbial biomass nitrogen  ninhydrin-reactive nitrogen  toluene treatment  volcanic ash soil
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