二穗短柄草被确定为水稻黑条矮缩病毒的新寄主

二穗短柄草(Brachypodium distachyon)是一种新兴的模式植物,在病毒-植物的互作研究中具有广阔的应用前景。水稻黑条矮缩病毒(Rice black-streaked dwarf virus,RBSDV)是一种重要的植物病毒,明确该病毒是否能够侵染二穗短柄草,是进行病毒-寄主互作研究的前提。本研究利用传毒介体灰飞虱将RBSDV人工接种于二穗短柄草Bd21,观察RBSDV是否侵染短柄草,以及侵染后的症状发展过程,同时对病毒进行了PCR检测。结果显示,RBSDV可以侵染二穗短柄草;初期症状为节间缩短,随后表现植株矮缩、心叶扭曲、缺刻等症状;PCR检测有明显的目的条带。由此确定二穗短柄草是RBSDV的新寄主,可作为该病毒与寄主互作的研究材料。这为进行RBSDV抗病基因鉴定、基因组学研究以及农作物的抗病育种奠定了基础。

Brachypodium distachyon,a New Host of Rice Black-Streaked Dwarf Virus

Brachypodium distachyon is a relatively ideal model plant for gramineous plants research at present.Rice black-streaked dwarf virus(RBSDV) is one of the most important virus in China, where it causes severe yield loss.Whether RBSDV infected Brachypodium distachyon or not, is the premise to establish virus-host interaction model. An inoculation method was developed to investigate the infection process and symptom development. Meanwhile, RBSDV was detected with PCR in B.distachyon.The results show that:B.distachyon could be infected by RBSDV. Typical symptoms such as stunting of plants, leaf distortion, small ears, outward-pointing notches could be observed in the plants. Furthermore, a clear single target band indicates that B.distachyon could be infected by RBSDV. According to these results, B.distachyon, a new host of RBSDV, can be used as a candidate to study virus-plant interactions.