胆囊收缩素主动免疫产蛋鸡的营养生理效应研究

用胆囊收缩素(CCK)主动免疫产蛋鸡，研究产蛋鸡血清和卵黄中CCK特异性抗体的产生规律及不同CCK免疫剂量对产蛋鸡生产性能和胰腺、胆囊发育及内分泌激素的影响。结果显示：用CCK-8主动免疫产蛋鸡，可以提高血清和卵黄CCK抗体水平，降低血液中CCK水平；CCK抗体的产生量在第1次加强免疫后即可接近高峰，第2、第3次加强免疫可维持较高的抗体水平。CCK主动免疫产蛋鸡后，对蛋鸡胰腺、胆囊发育、胰蛋白含量以及蛋鸡体内的生长激素、胰岛素和瘦素水平均没有显著影响，同时对蛋鸡采食量和料蛋比等生产性能也没有显著影响。用CCK主动免疫产蛋鸡，在不影响蛋鸡正常生理功能的同时，可以获得含特异性CCK抗体的卵黄。

Nutritional and Physiological Effects of Active Immunization against Cholecystokinin in Laying Hens

This study explored active immunization against cholecystokinin in laying hens and the effect on nutrition and physiology. The antibody titers of serum and yolk were examined by ELISA. The performance, nutrient digestibility,the development of pancreas and gallbladder, the activity of trypsin and the level of hormones after immunization were examined. The result showed active immunization against cholecystokinin resulted in development of high antibody titers in serum and yolk,decreased the level of cholecystokinin in blood.After the first booster injection, the antibody titers almost reached peak level.The development of gallbladder and pancreas were not affected by CCK immunization. The specific activity of pancreas-secreted enzymes was increased in treated group (CCK). Active immunization had no effect on growth hormone, insulin and leptin in serum. The performance of layers was not affected by CCK immunization. It is concluded that active immunization against cholecystokinin in laying hens has no negative impact on the physiology of hens and results in the production of eggyolk with high content of CCK antibody.