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| 黄瓜白粉病生防菌H_1、H_2的种类鉴定 | |
| 引用本文： | 范瑛阁,方海晶,韩鹏,张守村,韩松.黄瓜白粉病生防菌H_1、H_2的种类鉴定[J].甘肃农业大学学报,2013,48(2):50-54. |
| 作者姓名： | 范瑛阁 方海晶 韩鹏 张守村 韩松 |
| 作者单位： | 1. 塔里木大学植物科学学院,新疆阿拉尔843300;塔里木盆地生物资源保护利用兵团重点实验室,新疆阿拉尔843300 2. 霍城县农产品质量安全检测中心,新疆霍城,835200 3. 塔里木大学植物科学学院,新疆阿拉尔,843300 4. 塔里木盆地生物资源保护利用兵团重点实验室,新疆阿拉尔,843300 |
| 基金项目： | 国家自然科学基金:芽孢杆菌HH的种类鉴定及对黄瓜白粉病生防机理的研究 |
| 摘 要： | 通过形态特征、培养条件及生理生化指标,结合16SrDNA分子序列测定,对黄瓜白粉病生防菌H1、H2进行了鉴定.结果表明:生防菌H1、H2的形态特征及22项生理生化指标与枯草芽孢杆菌相符;生防菌H1、H216SrDNA序列与Genbank数据库中枯草芽孢杆菌的相似性高于99.5%,因此,H1、H2均为枯草芽孢杆菌亚种(Bacillus subtilis subsp.ubtilis).综合上述结果,最终确定生防菌H1、H2为枯草芽孢杆菌(Bacillus subtilis). |
| 关 键 词： | 黄瓜白粉病 枯草芽孢杆菌 生防菌 鉴定 |
Identification of biocontrol bacteria H1 and H2 against cucumber powdery mildew |
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| FAN Ying-ge , FANG Hai-jing , HAN Peng , ZHANG Shou-cun , HAN Song.Identification of biocontrol bacteria H1 and H2 against cucumber powdery mildew[J].Journal of Gansu Agricultural University,2013,48(2):50-54. | |
| Authors: | FAN Ying-ge FANG Hai-jing HAN Peng ZHANG Shou-cun HAN Song |
| Institution: | 1.College of Plant Science and Technology,Tarim University,Alar 843300,China;2.Xinjiang Production & Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin,Alar 843300,China;3.Agricultural Products Quality and Safety Testing Center of Huocheng County,Huocheng 835200,China) |
| Abstract: | Through the method of morphology,culture and physiological and biochemical determination,combined with 16S rDNA molecular sequence determination,the biocontrol bacterium H1 and H2 against cucumber powdery mildew were identified in this paper.The results show that the 22 morphological,physiological and biochemical indicators of biocontrol bacterium H1 and H2 are in line with the characteristics of Bacillus subtilis.The 16S rDNA molecular sequences of both are similar to Bacillus subtilis subsp.ubtilis.From the above results,biocontrol bacterium H1 and H2 are identified as Bacillus subtilis. |
| Keywords: | cucumber powdery mildew Bacillus subtilis biocontrol bacteria identification |
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