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| 高效液相色谱法同时测定马铃薯块茎中的GA_3、IAA和ABA含量 | |
| 引用本文： | 程建新,毕阳,田世龙,李梅,李守强. 高效液相色谱法同时测定马铃薯块茎中的GA_3、IAA和ABA含量[J]. 甘肃农业大学学报, 2013, 48(2) |
| 作者姓名： | 程建新 毕阳 田世龙 李梅 李守强 |
| 作者单位： | 1. 甘肃农业大学农学院,甘肃兰州730070;甘肃省农科院农产品贮藏加工研究所,甘肃兰州730070 2. 甘肃农业大学食品科学与工程学院,甘肃兰州,730070 3. 甘肃省农科院农产品贮藏加工研究所,甘肃兰州,730070 |
| 摘 要： | 用Agilent C18色谱柱(4.6mm×250mm),以乙腈、甲醇和0.6%乙酸VCH3CN∶VCH3OH∶V0.6%CH3COOH=5∶60∶35)为流动相,流速为0.8mL/min,在检测波长209、221、262nm,柱温30℃的条件下,同时分离并测定了马铃薯块茎中的内源激素GA3、IAA和ABA.3种激素的分离效果理想,回收率分别达到93.1%、90.3%和92.4%,相对标准偏差小于5.2%. |
| 关 键 词： | 高效液相色谱 内源激素 马铃薯 |
Simultaneous determination of GA3,IAA and ABA contents in potato tuber by high performance liquid chromatography |
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| CHENG Jian-xin , BI Yang , TIAN Shi-long , LI Mei , LI Shou-qiang. Simultaneous determination of GA3,IAA and ABA contents in potato tuber by high performance liquid chromatography[J]. Journal of Gansu Agricultural University, 2013, 48(2) | |
| Authors: | CHENG Jian-xin BI Yang TIAN Shi-long LI Mei LI Shou-qiang |
| Abstract: | A method for simultaneously determination and separation GA3,IAA and ABA in potato tibers was developed with a Agilent G1356D MWD detector,C18 chromatographic column(4.6 mm×250 mm),mobile phase formed acetonitrile,methanol and 0.6 % acetic acid(v/v ratio of 5∶60∶35),flow rate at 0.8 mL/min,wavelength at 209 nm,221 nm and 262 nm,and the column temperature at 30 ℃.GA3,IAA and ABA were well separated with their recoveries at 93.1%,90.3% and 92.4% respectively.RSD was below 5.2%. |
| Keywords: | high performance liquid chromatography endogenous hormone potato |
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