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一株牛源嗜酸性乳杆菌体外抑菌试验研究
引用本文:高淑琴,王庆奎,单晓枫,钱爱东.一株牛源嗜酸性乳杆菌体外抑菌试验研究[J].畜牧与饲料科学,2012(8):1-2,5.
作者姓名:高淑琴  王庆奎  单晓枫  钱爱东
作者单位:吉林农业大学动物科学技术学院;东辽县卫生职业技术学校;辽宁省盘山县高升镇动物卫生监督所
基金项目:吉林省畜牧局项目(吉牧科字第200905号)
摘    要:为了验证微生态活菌制剂候选菌株的益生特性,将筛选出的牛源嗜酸性乳杆菌NL0908进行体外抑菌试验,通过共培养和上清液抑菌试验,分析发现,菌株NL0908对病原菌大肠杆菌有较强的抑菌作用。将其上清液进行相应的热处理、蛋白酶处理、中和酸等,结果表明,其抑菌物质主要是由有机酸和抗菌小肽物质共同作用。

关 键 词:微生态活菌制剂  嗜酸性乳杆菌  体外抑菌  

Experimental Research on Bacteriostasis in vitro of Lactobacillus acidophilus from Calf Origin
GAO Shu-qin,WANG Qing-kui,SHAN Xiao-feng,QIAN Ai-dong.Experimental Research on Bacteriostasis in vitro of Lactobacillus acidophilus from Calf Origin[J].Animal Husbandry and Feed Science,2012(8):1-2,5.
Authors:GAO Shu-qin  WANG Qing-kui  SHAN Xiao-feng  QIAN Ai-dong
Institution:1 (1.Animal Science and Technology College, Jilin Agricultural University, Changchun 130118,China;2.Health Vocational School of Dongliao County, Dongliao 136600,China;3.Animal Health Supervision Agency of Gaosheng Town, Panshan Country, Liaoning Province, Panshan 124123,China)
Abstract:In order to verify probiotic properties of candidate strain, put selected NL0908 Lactobacillus acidophilus from calf origin into experiment of bacteriostasis in vitro, through co-culture and supernatant bacteriostatic test, it found that NL0908 strain pathogenic hadantibacterial activity to Escherichia coli. thermal, proteinase treatment and neutralization with supernatant, it proved that antibacterial substance may combined action organic acid and mini-peptides of antimicrobial substance.
Keywords:biogen  Lactobacillus acidophilus  bacteriostasis in vitro  calf
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