胡萝卜悬浮细胞系及高效再生系统的建立

以胡萝卜块根为材料诱导愈伤组织,并建立悬浮细胞系,然后将悬浮细胞和愈伤组织分别转入分化培养基进行分化培养。结果表明,胡萝卜块根形成层愈伤组织诱导率为100%,最佳激素种类和用量为2,4!D0.1mg/L;根据外观可将愈伤组织大致分为4种类型,I型是直接建立悬浮细胞系的良好来源,Ⅲ型经继代培养后可用于悬浮细胞系的建立;静止法是胡萝卜悬浮细胞系简单而有效的继代方法;胡萝卜愈伤组织分化的最佳培养基是MS或B5基本培养基,MS培养基上的再生苗可直接生根,而B5培养基上的再生苗在原培养基上很难生根,需要转移到MS培养基上才能生根。

Establishment of the Suspension Culture and Regeneration System of Carrot

The tap roots of carrot were used as the material to induce the calli and to establish the suspension cell culture system,then the calli and the cells of suspension culture were transferred on differentiation media for regeneration. The results showed that the inductivity of calli of carrot tap roots was 100 %,the best hormone and its concentration was 2,4-D and 0.1 mg/L.The calli could be approximately divided into four types by the external appearance: the type I was the optimal material for the establishment of the suspension cell culture,and the type Ⅲ could be used to establish the suspension cell culture after subculture.The absolute-rest precipitation method was found to be the simple and effective way to subculture the cell suspension system. The suitable medium for regeneration was MS or B5 basic medium without any hormone and the regenerated plant on MS could simultaneously develop the roots and shoot,the however,the rooting of the seedlings on B5 medium was very difficult on the original medium and must be transferred on MS medium to develop root.