| ‘石硖’龙眼芽变系双引物RAPD鉴定的研究 |
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| 引用本文： | 王心燕,肖璇,乔爱民,孙敏. ‘石硖’龙眼芽变系双引物RAPD鉴定的研究[J]. 园艺学报, 2006, 33(1): 134-136 |
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| 作者姓名： | 王心燕 肖璇 乔爱民 孙敏 |
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| 作者单位： | (仲恺农业技术学院园艺系, 广州510225; 江西赣南师范学院化学与生命科学系, 赣州341000; 西南师范大学生命科学学院, 重庆400715) |
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| 基金项目： | 广东省教育厅自然科学基金 |
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| 摘 要： | 以‘石硖’龙眼芽变系及其普通系(对照) 为材料, 从80对双引物中筛选出4对双引物在二者之间扩增出稳定清晰的多态性片段。4对双引物共扩增出35条带, 其中多态性带5条, 表明通过双引物扩增可成功地将‘芽变’与母株(对照) 区分开来。在此基础上对双引物产生多态性扩增的原理进行了分析。
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| 关 键 词： | 龙眼 芽变 RAPD 双引物 |
| 文章编号： | 0513-353X（2006）01-0134-03 |
| 收稿时间： | 2005-01-25 |
| 修稿时间： | 2005-01-252005-07-22 |
RAPD Studies on Sport Line of ''''Shixia'''' Longan with Double Primers |
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| Wang Xinyan,Xiao Xuan,Qiao Aimin,Sun Min. RAPD Studies on Sport Line of ''''Shixia'''' Longan with Double Primers[J]. Acta Horticulturae Sinica, 2006, 33(1): 134-136 |
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| Authors: | Wang Xinyan Xiao Xuan Qiao Aimin Sun Min |
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| Affiliation: | (Departm ent of Horticulture, Zhongkai University of Agriculture and Technology, Guangzhou 510225, China; Department of Chemistry and Life Science, Gannan Normal University, Ganzhou 341000, China; College of Life Science, Southwest China Normal University, Chongqing 400715, China) |
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| Abstract: | A single 10 bp random oligonucleotide was often used as the primer in the standard RAPD-PCR analysis.To identify the possibility of RAPD-PCR reaction with double 10 bp primers,80 double 10 bp oligonucleotides were applied to the RAPD-PCR analysis between the'Shixia'longan(Dimocarpus longan var.shixia Lour.) sport line and it's normal line(control).Four double primers which could produce clear and stability polymorphic DNA fragments were screened.Five RAPD makers have been obtained which were linked to the mutation gene of the sport line.Furthermore,the reason why the new RAPD fragments could be produced by double primers was also discussed. |
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| Keywords: | Longan Sport RAPD Double primers |
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